AGING AND ESTROGEN ON BIOGENIC AMINE PHYSIOLOGY-RCDA

该实验室正在利用细胞电生理学方法研究 女性生殖衰老的中枢调节。除了……之外 参与管理和教学两个方面的医学 和研究所的研究生院项目，她的研究包括 人参皂苷的细胞生理和药理作用研究 雌激素、5-羟色胺和去甲肾上腺素对大鼠神经元电活动的影响 已确定的含5-羟色胺和去甲肾上腺素的细胞群(背部 分别为中缝核和蓝斑)，它们可能介导雌性 生殖衰老过程。年轻、中年和有生殖能力的自行车 正在老化的雌性费舍尔344只大鼠被用来研究 神经元事件的细胞生理学，这可能是 随着年龄的增长，受体的功能发生了变化。 这位基础研究科学家的长期职业目标包括 继续追求神经内分泌学的基础知识 中心老化过程，如在学术环境中进行。 重要的是，这位调查员希望扩大她的研究重点 女性生殖衰老的研究将包括分子 生理和神经化学方法。简要概述，建议的 研究培训将包括膜生物物理学和膜片钳 在包括细胞在内的各种制剂中应用的记录技术 培养，分离细胞，暴露在脑片中的神经元。建议数 分子神经化学培训将强调原位使用 杂交技术在第二信使调控中的应用 神经递质功能。对这项研究计划的支持对于 她作为一名神经学家的持续发展和对 她在神经内分泌学研究方面的当代问题 衰老的问题。这项奖励将为她提供几乎100%的时间 去研究和研究训练。拉科斯基博士的机构全力支持 拟议的神经药理学分子方面的培训计划 并将根据奖励条款重新分配她的时间。

The laboratory is utilizing cellular electrophysiologic approaches to study the central regulation of female reproductive aging. In addition to participation in both administration and teaching aspects of the Medical and Graduate School Programs of her institution, her research includes studies on the cellular physiological and pharmacological effects of estrogen, serotonin and norepinephrine on the neuronal activity of identified serotonin- and norepinephrine-containing cell groups (dorsal raphe nucleus and locus coeruleus, respectively) which may mediate female reproductive aging processes. Cycling young, middle-aged and reproductively senescent female Fischer 344 rats are being utilized to investigate the cellular physiology of neuronal events which may be a consequence of altered receptor function with aging. The long term career goals of this basic research scientist include continued pursuit of fundamental knowledge of the neuroendocrinology of central aging processes, as carried out in an academic setting. Importantly, this investigator wishes to expand the focus of her investigations of female reproductive aging to include molecular physiological and neurochemical approaches. Briefly outlined, the proposed research training will encompass membrane biophysics and patch-clamp recording techniques as applied in a variety of preparations including cell cultures, isolated cells and exposed neurons in brain slices. The proposed molecular neurochemical training will emphasize use of in situ hybridization techniques as applied to second messenger modulation of neurotransmitter function. Support of this research plan is essential to her continued development as a neuroscientist and for investigation of contemporary problems in her research expertise in the neuroendocrinology of aging. This award will provide for almost 100% of her time to be devoted to research and research training. Dr. Lakoski's institution fully supports the proposed plan for training in molecular aspects of neuropharmacology and will redistribute her time in accord with the terms of the award.