AGING AND ESTROGEN ON BIOGENIC AMINE PHYSIOLOGY-RCDA

该实验室正在利用细胞电生理学方法研究 女性生殖衰老的中枢调节除了 参与医疗管理和教学工作 和她所在机构的研究生院课程，她的研究包括 细胞生理和药理作用的研究 雌激素、5-羟色胺和去甲肾上腺素对 鉴定的含5-羟色胺和去甲肾上腺素的细胞群（背侧 中缝核和蓝斑），可能介导女性 生殖老化过程。骑自行车年轻，中年和生殖 衰老的雌性Fischer 344大鼠被用于研究 神经元事件的细胞生理学，其可能是 随着年龄的增长而改变受体功能。 这位基础研究科学家的长期职业目标包括 继续追求神经内分泌学的基础知识， 在学术环境中进行的中央老化过程。 重要的是，这位调查员希望扩大她的关注范围， 女性生殖老化的调查，包括分子 生理学和神经化学方法。简要概述了拟议的 研究培训将包括膜生物物理学和膜片钳 记录技术应用于各种制剂， 培养物、分离的细胞和脑切片中暴露的神经元。拟议 分子神经化学训练将强调使用原位 应用于第二信使调节的杂交技术 神经递质功能支持这项研究计划是必不可少的， 她作为一名神经科学家的持续发展， 她在神经内分泌学研究专长中的当代问题 衰老的迹象这个奖项将提供她几乎100%的时间投入 to research研究and research训练.拉科斯基医生的机构全力支持 拟议的神经药理学分子方面的培训计划 并将重新分配她的时间在雅阁与奖励的条款。

The laboratory is utilizing cellular electrophysiologic approaches to study the central regulation of female reproductive aging. In addition to participation in both administration and teaching aspects of the Medical and Graduate School Programs of her institution, her research includes studies on the cellular physiological and pharmacological effects of estrogen, serotonin and norepinephrine on the neuronal activity of identified serotonin- and norepinephrine-containing cell groups (dorsal raphe nucleus and locus coeruleus, respectively) which may mediate female reproductive aging processes. Cycling young, middle-aged and reproductively senescent female Fischer 344 rats are being utilized to investigate the cellular physiology of neuronal events which may be a consequence of altered receptor function with aging. The long term career goals of this basic research scientist include continued pursuit of fundamental knowledge of the neuroendocrinology of central aging processes, as carried out in an academic setting. Importantly, this investigator wishes to expand the focus of her investigations of female reproductive aging to include molecular physiological and neurochemical approaches. Briefly outlined, the proposed research training will encompass membrane biophysics and patch-clamp recording techniques as applied in a variety of preparations including cell cultures, isolated cells and exposed neurons in brain slices. The proposed molecular neurochemical training will emphasize use of in situ hybridization techniques as applied to second messenger modulation of neurotransmitter function. Support of this research plan is essential to her continued development as a neuroscientist and for investigation of contemporary problems in her research expertise in the neuroendocrinology of aging. This award will provide for almost 100% of her time to be devoted to research and research training. Dr. Lakoski's institution fully supports the proposed plan for training in molecular aspects of neuropharmacology and will redistribute her time in accord with the terms of the award.