DEFINING MUCOSAL LYMPHOCYTE SUBPOPULATION

炎症性肠病、溃疡性结肠炎和克罗恩病 疾病的组织学特征是淋巴细胞聚集 和中性粒细胞固有层和灶内的浆细胞 淋巴细胞。长期以来，免疫机制一直被怀疑存在于 IBD的发病机制尚不清楚。它也不是 明确淋巴细胞数量或功能的改变 介导粘膜损伤或者是炎症的附属物。当前 对肠道功能特性的研究进展 粘膜淋巴细胞亚群，尤其是上皮内 淋巴细胞(IEL)，由于不能特异性地 从肠道标本分离后鉴定细胞悬液中的IEL。 这个项目的主要目标是定义可能的表型和 IBD和IBD患者粘膜淋巴细胞亚群的功能差异 正常的肠道。这将通过使用最近开发的 主要局限于粘膜淋巴细胞的单抗 尤其是IEL。识别T细胞的单抗 受体伽马/增量也将被用来确定肠道 这些细胞在IBD中的分布及其功能特点。一个 儿童淋巴细胞亚群分布或功能的差异 固有层或上皮室可能在 IBD的发病机制。

The inflammatory bowel diseases (IBD), ulcerative colitis and Crohn's disease, are histologically characterized by an accumulation of lymphocytes and plasma cells in the lamina propria and foci of polymorphonuclear lymphocytes. An immune mechanism has long been suspected in the pathogenesis of IBD but, the etiology remains unknown. It is also not clear whether alterations in lymphocyte populations or their function mediate the mucosal injury or are epiphenomena of inflammation. Current research efforts investigating the functional properties of intestinal mucosal lymphocyte subpopulations, especially the intraepithelial lymphocytes (IEL), have been hampered by the inability to specifically identify IEL in cell suspension after isolation from intestinal specimens. The major goal of this project is to define possible phenotypic and functional differences in the mucosal lymphocyte subpopulations in IBD and normal intestine. This will be accomplished by using recently developed monoclonal antibodies that are primarily restricted to mucosal lymphocytes and IEL in particular. Monoclonal antibodies that recognize the T cell receptor gamma/delta will also be used to determine the intestinal distribution of these cells in IBD and their functional characteristics. A difference in the distribution or function of lymphocyte subpopulations in the lamina propria or epithelial compartments, may play a role in the pathogenesis of IBD.