REGULATION OF RENAL MEMBRANE PERMEABILITY

这项提议将检验膜电导率 通过细胞内信使的介导进行调节， 并且这种调节会影响细胞 膜电位和产电膜转运 流程. 具体而言，此应用程序将评估 腺苷酸环化酶/cAMP介导的信使途径和 肌醇磷脂信使途径在膜调节中作用 刷状缘的电导和膜运输， 大鼠肾近曲小管基底外侧膜囊泡。 此外，该提案将决定是否在实质上 这些信使途径的诱导变化导致 渗透性、离子电导和转运 近端小管的曲节和直节， 来自这些异质肾单位的膜泡 网站. 为了实现这些目标，膜渗透性和 电导将用荧光和同位素 本实验室开发的检测方法， 将使用特定的底物进行检查， 在刷状缘或 基底外侧膜

The proposal will test the hypothesis that membrane conductance is regulated through the mediation of intracellular messengers, and that this regulation secondarily affects both the cell membrane potential and electrogenic membrane transport processes. Specifically, this application will assess the role of the adenylate cyclase/cAMP mediated messenger pathway and the inositol phospholipid messenger pathway in regulating membrane conductances and membrane transport in brush border and basolateral membrane vesicles of rat renal proximal tubule. Additionally, this proposal will determine whether hormonally induced changes in these messenger pathways result in changes in permeability, ionic conductances and transport in isolated convoluted and straight segments of proximal tubules, and in membrane vesicles derived from these heterogeneous nephron sites. To accomplish these goals, membrane permeabilities and conductances will be evaluated with the fluorescent and isotopic assays that have been developed in this laboratory, and transport will be examined utilizing specific substrates that are electrogenically transported in either the brush border or basolateral membranes.