RENAL RESPONSES TO CHANGES IN PHOSPHATE SUPPLY

Scheinman博士是一名肾病学家，他的教师职业生涯始于助理 纽约州立大学医学教授北部医疗中心 他获得 M.D.耶鲁大学内科学学位和住院医师培训， 在上州和耶鲁大学接受肾脏学专科培训 期间 研究训练，他适应了孤立的灌流大鼠肾脏作为一个 甲状旁腺激素作用的体外模型。 他的目标是建立 作为一个独立的实验室研究员，他建议 初步研究一个实质性的未解决的问题，在监管 无机磷酸盐（Pi）体内平衡：改变信号的性质 体内Pi供应变化期间肾脏Pi的重吸收。 拟议 研究将检查（1）Pi是否可以直接影响肾脏Pi 运输;（2）细胞外或饮食pi与 肾细胞内Pi;以及（3）存在肾外信号 控制肾Pi重吸收。 将研究Pi的直接作用 使用一种新的模型来研究肾脏转运的调节：大鼠 肾脏将在体外用各种组成的培养基灌注， 将从这些肾脏中制备BBMV并进行研究。 细胞内Pi将是 在灌注的肾脏中，可能在体内的肾脏中，使用新的 31P-NMR波谱技术。 最后，将建立一个模型， 肾对肠外营养期间Pi供应变化的适应性 大鼠，该模型将用于确定 胃肠道在这种肾脏适应。 这些研究可能 最终揭示了Pi体内平衡的各种条件， 紊乱，可能包括维生素D抵抗性低磷酸盐血症 佝偻病、肾功能不全和某些形式的肾结石病。 博士欧文M.韦纳将担任赞助商，乔治C博士。莱维作为 二级申办者将指导NMR研究。 李博士，博士。 沙因曼就能进入国家卫生研究所多核NMR资源和 数据处理位于锡拉丘兹大学。 玛丽艾伦特林布尔博士 将合作进行BBMV研究，迈克尔·马吉德博士将 合作研究父母营养问题。 此外该 纽约州立大学北部医学中心有许多活跃在肾脏生理学领域的科学家 以及跨学科咨询的悠久传统。

Dr. Scheinman is a nephrologist beginning his faculty career as Assistant Professor of Medicine at S.U.N.Y.-Upstate Medical Center. He received his M.D. degree and residency training in Internal Medicine at Yale, and nephrology subspecialty training at both Upstate and Yale. During his research training he adapted the isolated perfused rat kidney for use as an in vitro model of parathyroid hormone effects. His goal is to establish himself as a major independent laboratory investigator, and he proposes initially to study a substantial unsolved problem in the regulation of inorganic phosphate (Pi) homeostasis: the nature of the signal that alters renal Pi reabsorption during changes in body Pi supply. The proposed studies will examine (1) whether Pi can directly influence renal Pi transport; (2) the relationship between extracellular or dietary pi and renal intracellular Pi; and (3) the existence of an extrarenal signal governing renal Pi reabsorption. A direct role of Pi will be examined using a new model for studying the regulation of renal transport: rat kidneys will be perfused in vitro with media of various compositions, and BBMV will be made from these kidneys and studied. Intracellular Pi will be assessed in perfused kidneys, and possibly kidneys in vivo, using the new technique of 31P-NMR spectroscopy. Finally, a model will be developed of renal adaptation to changes in Pi supply during parenteral nutrition in rats, and this model will be used to determine the importance of the gastrointestinal tract in this renal adaptation. Such studies may eventually shed light on a variety of conditions in which Pi homeostasis is disordered, possibly including vitamin D-resistant hypophosphatemic rickets, renal insufficiency, and certain forms of renal stone disease. Dr. Irwin M. Weiner will serve as sponsor, and Dr. George C. Levey as secondary sponsor will direct the NMR studies. Through Dr. Levy, Dr. Scheinman will have access to the N.I.H. Resource for Multinuclei NMR and Data Processing located at Syracuse University. Dr. Mary Ellen Trimble will collaborate on the BBMV studies, and Dr. Michael Meguid will collaborate on studies involving parental nutrition. In addition, the SUNY-Upstate Medical Center has many scientists active in renal physiology and a long tradition of interdisciplinary consultation.