CALCIUM CHANNEL TYPES IN THE DEVELOPING HUMAN BRAIN

电压敏感钙通道作为电信号的换能器 事件转化为化学事件在中枢神经系统中具有独特的作用 功能。了解这一渠道的发展对于 许多疾病的病理生理学，例如缺氧缺血神经细胞 年第一年的损害和癫痫发生率的增加 生活。利用来自圆锥海螺的独特的新神经肽探针， 检测钙通道的数量、类型和结构的变化 研究开发人脑病理标本。毒素结合， 阻断突触体钙摄取及凝胶电泳法 与毒素的交联会被用来表征钙通道。数据来自 鸡和老鼠的大脑样本将与人类大脑的结果进行比较。这个 兴奋性神经递质谷氨酸AS的电压敏感性释放 以及钙通道阻滞剂的药理作用。 谷氨酸的释放也将被研究。

The voltage sensitive calcium channel as the transducer of electrical events into chemical events has a unique role in central nervous system function. Understanding the development of this channel is essential to the pathophysiology of many diseases, for example hypoxic-ischemic neuronal damage and the increased incidence of seizure during the first year of life. Using unique new neuropeptide probes from the Conus marine snail, detection of changes in calcium channel number, type and structure will be studied in developing human brain pathology specimens. Toxin binding, block of calcium45 uptake in synaptosomes and gel electrophoresis after crosslinking to toxin will be used to characterize Ca channels. Data from chick and mouse brain samples will be compared to human brain results. The voltage sensitive release of the excitatory neurotransmitted glutamate as well as the pharmacologic effect of the calcium channel blocker on glutamate release will also be studied.