CALCIUM CHANNEL TYPES IN THE DEVELOPING HUMAN BRAIN

发育中的人脑中的钙通道类型

• 批准号: 3081423
• 负责人: MARCIA LITZINGER
• 金额: $ 6.7万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-12-01 至 1992-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3081423
• 关键词: biological signal transduction; calcium channel; calcium channel blockers; calcium flux; chemical binding; chickens; crosslink; developmental neurobiology; gel electrophoresis; glutamates; human tissue; laboratory mouse; neuropeptides; neuropharmacology; neurotransmitters; protein structure; synapses; synaptosomes; toxin

The voltage sensitive calcium channel as the transducer of electrical events into chemical events has a unique role in central nervous system function. Understanding the development of this channel is essential to the pathophysiology of many diseases, for example hypoxic-ischemic neuronal damage and the increased incidence of seizure during the first year of life. Using unique new neuropeptide probes from the Conus marine snail, detection of changes in calcium channel number, type and structure will be studied in developing human brain pathology specimens. Toxin binding, block of calcium45 uptake in synaptosomes and gel electrophoresis after crosslinking to toxin will be used to characterize Ca channels. Data from chick and mouse brain samples will be compared to human brain results. The voltage sensitive release of the excitatory neurotransmitted glutamate as well as the pharmacologic effect of the calcium channel blocker on glutamate release will also be studied.

电压敏感性钙通道作为电传导器 事件转化为化学事件在中枢神经系统中具有独特的作用 功能 了解这一渠道的发展对于 许多疾病的病理生理学，例如缺氧缺血性神经元 第一年缉获发生率的增加 生活 使用来自芋螺的独特的新神经肽探针， 检测钙通道数量、类型和结构的变化将是 研究人类大脑病理学标本。 毒素结合， 阻断突触体中钙45的摄取和凝胶电泳 与毒素的交联将用于表征Ca通道。 数据从 鸡和小鼠脑样品将与人脑结果进行比较。 的 兴奋性神经传递谷氨酸的电压敏感性释放， 以及钙离子通道阻滞剂的药理作用， 还将研究谷氨酸盐释放。