CELLULAR TROPISM OF HIV

这一医生/科学家奖旨在提供严格的 和全面的科学培训，为在中国的职业生涯做准备 具有扎实的基础科学研究承诺的学术医学。 它结合了怀特黑德大学一流的学术氛围 麻省理工学院，哈佛大学 医学院和马萨诸塞州综合医院(MGH)。这个 第一阶段赞助商大卫·巴尔的摩博士有一个令人印象深刻的 对培养和培训年轻医生的热情/ 科学家。 第一阶段将包括一次密集的研究体验 怀特黑德研究所。同时，研究生水平的课程工作 在分子遗传学方面，分子病毒学和免疫学将 在哈佛大学、麻省理工学院和海洋生物实验室进行 在伍兹霍尔。在大卫·巴尔的摩博士的实验室里，这项研究 将重点介绍对人类免疫缺陷病毒的理解 (HIV)在单核/巨噬细胞中复制。极致的 将使用凝胶研究艾滋病毒复制的复杂性质 研究细胞特异性的滞留试验和DNA保护试验 参与指导HIV蛋白转录的因素。 一种重要的细胞衍生因子，核因子Kappa B，已被证明 要参与艾滋病毒在T细胞系中的复制，将进行研究 在单核/巨噬细胞中。我们还将调查是否有 嗜性细胞与单核/巨噬细胞是否存在遗传差异 热带艾滋病病毒株。限制性内切酶分析、克隆和 不同细胞HIV特异性分离株的核苷酸序列分析 将进行定向检查，以检查是否存在特定的 HIV毒株在长末端重复序列内的遗传差异 和/或包膜糖蛋白区域。严谨的理解 单核/巨噬细胞中HIV的产生将是关键 了解该病的多种致病机制 病毒。在此期间，应聘者将熟悉 一些重要的和可推广的分子病毒学 技巧。

This Physician/Scientist Award is designed to provide a rigorous and thorough scientific training in preparation for a career in academic medicine with intensive basic science research commitment. It combines the superb academic atmosphere at the Whitehead Institute, Massachusetts Institute of Technology, the Harvard Medical School and the Massachusetts General Hospital (MGH). The Phase One sponsor, Dr. David Baltimore, has an impressive enthusiasm for the nurture and training of young physician/ scientists. Phase One will involve an intensive research experience at the Whitehead Institute. At the same time, graduate level course work in molecular genetics, molecular virology and immunology will be conducted at Harvard, M.I.T. and the Marine Biological Laboratory at Woods Hole. In Dr. David Baltimore's laboratory, the research will focus on the understanding of Human immunodeficiency Virus (HIV) replication in the monocyte/macrophage. The extremely complex nature of HIV replication will be investigated using gel retardation assays and DNA protection assays to study cell-specific factors involved in directing the transcription of HIV proteins. An important cell-derived factor, NF Kappa B, which has been shown to be involved in HIV replication in T cell lines, will be studied in the monocyte/macrophage. We will also investigate whether there are genetic differences in a cell tropic versus monocyte/macrophage tropic HIV strains. Restriction enzyme analysis, cloning and nucleotide sequencing of specific HIV isolates with differing cell tropism will be performed to examine whether there exist specific genetic differences in HIV strains within the long terminal repeat and/or the envelope glycoprotein region. A rigorous understanding of HIV production in the monocyte/ macrophage will be key in understanding a variety of the pathogenetic mechanisms of this virus. During this time, the candidate will become familiar with a number of important and generalizable molecular virologic techniques.