STUDIES ON GENE EXPRESSION DURING GRANULOPOIESIS

本申请书旨在申请一笔研究培训补助金，使申请人能够 在蛋白质化学、免疫化学和细胞方面获得研究经验 生物学，同时延长了他之前在重组 DNA方法，通过对基因表达调控的研究 在粒细胞生成过程中。研究计划分为两个阶段。 在第一阶段，申请者打算在Frank博士那里进行研究 Ruddle实验室对转铁蛋白受体基因的调节 早幼粒细胞白血病细胞系。DNA-蛋白质印迹技术 拉德尔博士实验室的最新研发成果将被用来 鉴定调节转铁蛋白转录的蛋白质 受体基因。第二阶段将在爱德华·本茨博士的实验室进行 并将包括对基因调控的研究，而不是 粒细胞成熟阶段的转铁蛋白受体基因 例如，一个早幼粒白血病细胞系被诱导为 末端成熟。调控这些基因转录的蛋白质 将被描述为。这些研究预计将为申请者提供 在分子生物学的所有领域进行广泛的培训，同时 提供有关粒细胞生成调节的启发性信息。

This application is for a research training grant to allow the applicant to gain research experience in protein chemistry, immunochemistry and cell biology and at the same time extend his previous training in recombinant DNA methods, by conducting research on the control of gene expression during granulopoiesis. The research program is divided into two phases. In Phase I the applicant intends to carry out research in Dr. Frank Ruddle's laboratory on the regulation of the transferrin receptor gene in a promyelocytic leukemia cell line. A DNA-protein blotting procedure recently developed in Dr. Ruddle's laboratory will be utilized to characterize proteins that regulate transcription of the transferrin receptor gene. Phase II will be spent in the laboratory of Dr. Edward Benz and will consist of studies on the regulation of genes other than the transferrin receptor gene during the maturation phase of granulopoiesis as exemplified by the induction of a promyelocytic leukemia cell line to terminal maturation. Proteins regulating the transcription of these genes will be characterized. These studies are expected to give the applicant broad training in all areas of molecular biology and at the same time produce illuminating information on the regulation of granulopoiesis.