NHLBI MINORITY SCHOOL DEVELOPMENT AWARD

EGR-L、EGR2和EGR3是早期反应基因，这意味着它们是 有丝分裂原、生长因子和 各种其他刺激物。它们也是含锌指的 与序列GCGGGGCG特异结合的转录因子，a 特异性是由两个相关因素所共有的，NGF1-C和 Wilm‘s肿瘤基因的产物，WT33。有证据表明 L参与建立和保持差异化 在心肌和I类神经细胞中的功能。 这项研究项目的目标是：1)确定 EGR样转录因子的表达对生长至关重要 控制和分化，并确定潜在的靶基因。2)至 确定EGR-L特定蛋白结构域的功能。3)至 描述并确定翻译后翻译的功能 L对废气再循环的修改。在这些实验中获得的结果应该 帮助阐明分化途径是如何确定的以及如何 某些组织的分化功能保持不变。这项研究 还应有助于更好地了解 转录调控因子。 如果获奖，拟议的拨款将支持和维持一项研究 梅哈里医学院致力于转录领域的研究 监管。这将扩大现有的培训机会。 生物化学系的少数民族学生。正式的 建议与范德比尔特大学达里尔·格兰纳博士建立关系 将提供互惠的培训机会和技术转让 在这两所学校之间。

Egr-l, EGR2, and EGR3 are early response genes, meaning that they are rapidly and transiently stimulated by mitogens, growth factors, and a variety of other stimuli. They are also zinc finger containing transcription factors which bind specifically to the sequence GCGGGGCG, a specificity which is shared by two related factors, NGF1-C, and the product of the Wilm's tumor locus, WT33. There is evidence to suggest that egr-l is involved in establishing and maintaining differentiated function in cardiac muscle and I neural cell types. The goals of this research project are: 1) To determine whether the expression of egr-like transcription factors are critical to growth control and differentiation and to identify potential target genes. 2) To determine the function of particular protein domains of egr-l. 3) To characterize and determine the function of post-translational modifications of egr-l. Results obtained in these experiments should help elucidate how differentiation pathways are determined and how differentiated function of certain tissues is maintained. This research should also lead to a greater understanding of the egr family of transcriptional regulators. If awarded, the proposed grant will support and maintain a research effort at Meharry Medical College focused on the field of transcriptional regulation. This will broaden the available opportunities for training minority students in the Biochemistry Department. The formal relationship proposed with Dr. Daryl Granner of Vanderbilt University will afford reciprocal training opportunities and technology transfer between the two schools.