ROLE OF THROMPOSPONDIN IN ODONTOGENESIS

这一奖项的目的是为申请者提供机会 获得成为一名完全独立的生物医学医生所需的技能 具有分子生物学应用专业知识的研究人员 上皮间充质组织病理学问题的技巧 互动。有了这些技能，申请者将应用他的培训 致力于颅面发育和口腔病理学的研究 在牙科学术领域发展事业。颁奖的初始阶段 将在跨学科项目中用于教学研究 密歇根大学细胞和分子生物学 实验室轮换学习不同的分子生物学技术 (RFLP分析、转基因小鼠程序、克隆技术、网站 定向突变，在哺乳动物细胞中的表达，原位 胚胎学中靶蛋白的杂交、免疫定位 纸巾)。最后阶段将包括这些研究的应用。 研究细胞外细胞黏附蛋白作用的工具 (血栓反应蛋白)在牙齿形成中的作用。这项研究的目的是整合 凝血酶原蛋白在快速增长的知识中的作用 细胞黏附蛋白、其他细胞外基质蛋白和细胞 上皮-间充质相互作用中的跨膜受体 发生在牙齿形成过程中。由于牙齿的形成是一个研究 基因调节依赖器官的空间和时间形成的方式 关于不同胚胎细胞系的相互作用，获得的信息 从这个模型应该适用于开发(正常和 异常的)其他器官系统的。

The purpose of this award is to provide the applicant with the opportunity to obtain the skills necessary to become a fully independent biomedical investigator with expertise in the application of molecular biological techniques to questions of the pathology of epithelial-mesenchymal interactions. With these skills, the applicant will apply his training towards research in craniofacial development and oral pathology while developing a career in academic dentistry. The initial phase of the award will be spent in didactic study within the interdisciplinary program in Cellular and Molecular Biology at the University of Michigan and in laboratory rotations learning different techniques of molecular biology (RFLP analysis, transgeneic mouse procedures, cloning technology, site directed mutagenesis, cDNA expression in mammalian cells, in-situ hybridization, immunolocalization of target proteins in embryologic tissues). The final phase will consist of application of these research tools to investigate the role of an extracellular, cell adhesion protein (thrombospondin) in odontogenesis. The aim of this research is to integrate the role of thrombospondin to the rapidly expanding knowledge available on cell adhesion proteins, other extracellular matrix proteins, and cellular transmembrane receptors in the epithelial-mesenchymal interactions occurring in tooth formation. Since tooth formation is a model to study the way genes regulate the spatial and temporal formation of organs dependent on the interaction of different embryonic cell lines, information obtained from this model should be applicable to the development (normal and aberrant) of other organ systems.