BIOGENESIS OF CANALICULAR MEMBRANE GLYCOPROTEINS

这项研究提案描述了一项计划，以表征 一种大鼠肝膜糖蛋白的细胞内生物合成途径 与牛磺胆酸胆汁酸转运有关，并存在于 位于顶端或胆小管膜处的极化肝细胞。这个 该分子的生物发生将使用代谢标记进行分析 结合亚细胞分离、免疫吸附和 免疫印迹。通过这种方法，牛磺胆酸盐转运体 会从高尔基体中的糖基转移到正弦 然后到达胆小管的膜，在那里这个 糖蛋白发挥其功能。要回答的问题是 “这种在胆汁中起作用的糖蛋白在什么时候起作用？ 小管，从具有其他功能的糖蛋白中分离出来 是否仅限于正弦膜？“回答这个问题 将澄清我们对细胞内糖蛋白生物发生的理解 并为未来对 肝胆疾病的发病机制。

This research proposal describes a plan to characterize the intracellular biosynthetic pathway of a rat liver membrane glycoprotein implicated in taurocholate bile acid transport and present in the polarized hepatocyte at the apical or bile canalicular membrane. The biogenesis of this molecule will be analyzed using metabolic labeling in combination with subcellular fractionation, immunoadsorption and immunoblotting. With such an approach, the taurocholate transporter will be followed from its glycosylatin in the Golgi to the sinusoidal membrane and then to the bile canalicular membrane where this glycoprotein carries out its unction. The question tap be answered is "At what point does this glycoprotein, which functions at the bile canaliculus, sort itself from glycoproteins with other functions that are restricted to the sinusoidal membrane?" Answers to this question will clarify our understanding of intracellular glycoprotein biogenesis and establish a foundation for future investigation into the pathogenesis of hepatobiliary disease.