BIOPHYSICAL STUDIES OF EPITHELIAL ION TRANSPORT

该建议是为了支持在联合国系统内开展一项全面的培训方案， 膜研究的基本生理学最新技术水平 上皮转运 具体而言，该项目的目的是研究 离子通道的电特性和产电转运 肾小管上皮细胞膜中的系统。 方法 用于研究可兴奋的组织制备物， 单个单位电流的测量将应用于上皮细胞 用膜片钳技术记录细胞膜。 该项目将涉及 分析顶端通道和产电运输系统在 近端小管的腔膜。 此外，方法将 为直接研究近端基底外侧膜而开发 小管 最初的实验将全部在Ambystoma的细胞上进行 （蝾螈）肾，一个物种与大细胞大小，从而允许 膜片钳技术与经典膜片钳技术的比较 电生理测量。 在第二阶段，这些技术 将扩展到哺乳动物肾小管制剂。 该项目的结果预计将提供新的信息， 传统的单电池无法实现的运输系统 肾脏电生理学中使用的穿刺技术。

The proposal is for support of a comprehensive training program in the basic physiological state of the art techniques in membrane studies of epithelial transport. Specifically the aims of the project are to study the electrical characteristics of ion channels and electrogenic transport systems in epithelial cell membranes of the renal tubule. Methods introduced for the study of excitable tissue preparations and the measurements of single unit currents will be applied to epithelial cell membranes using the patch-clamp technique. The project will involve the analysis of apical channels and electrogenic transport systems in the luminal membrane of the proximal tubule. In addition, methods will be developed for the direct study of the basolateral membrane of the proximal tubule. Initial experiments will all be performed on cells of Ambystoma (salamander) kidney, a species with large cell size, thus allowing for comparison between patch-clamp measurements and classical electrophysiological measurements. In a second phase, these techniques will be extended to mammalian renal tubule preparations. The results of this project are expected to provide new information about transport systems which cannot be obtained with traditional single-cell impalements techniques used in electrophysiology of the kidney.