POWRE: The Evolving Function of the Basolateral Membrane Ca Pump in Exchange Epithelia of a Continuum of Aquatic to Semi-Terrestrial Brazilian Crabs

POWRE：水生到半陆生巴西蟹连续体交换上皮细胞中基底外侧膜钙泵的进化功能

• 批准号: 9870374
• 负责人: Michele Wheatly
• 金额: $ 7.5万
• 依托单位: Wright State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-11-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9870374&HistoricalAwards=false
• 关键词: POWRE; Evolving; Function; Basolateral; Membrane

Wheatly 9870374 The grant will allow the PIs to assess how calcium transporting proteins evolved. The PIs will study the physiological function and molecular characterization of the calcium pump in transporting epithelia (gills, kidney, gut) of an evolutionary continuum of related aquatic to semi-terrestrial crabs available in Brazil. Crustaceans are used as a non-mammalian model system for the study of calcium balance due to the mineralization/demineralization processes that accompany their natural molting cycle. The hypothesis is that during evolution from water onto land aquatic modes of Calcium uptake (across gills) become superseded by terrestrial modes (dietary intake across gut). The specific objectives are to examine the Ca uptake proteins in the membrane of gill, kidney and gut in each crab as a function of (a) stage in the molting cycle and (b) CA availability (water or dietary). Methods will include making membrane vesicles (analogous to water filled balloons) to study Ca uptake in vitro as well as using molecular biology to clone and sequence tissue-specific genes. Expression of the genes will be quantified during molting and as a function of Ca availability. The Ca pump is fundamental to Ca homeostasis in all eukaryotic cells. This research will delineate the evolution of this critical molecule over the past 600 million years.

9870374美元的拨款将允许PI评估钙转运蛋白是如何进化的。PIS将研究钙泵在运输上皮(鳃、肾、肠道)中的生理功能和分子特征，这些上皮是巴西现有的水生到半陆生螃蟹的进化连续体。甲壳动物由于其自然蜕皮过程中的矿化/脱矿过程，被用作研究钙平衡的非哺乳动物模型系统。假设是，在从水到陆地的进化过程中，水中钙摄取的模式(通过鳃)被陆地模式(通过肠道的饮食摄取)所取代。具体目标是研究每只蟹鳃、肾和肠道膜上钙吸收蛋白随(A)蜕皮周期阶段和(B)钙有效性(水或饲料)的变化。方法将包括制造膜泡(类似于充满水的气球)来研究体外钙吸收，以及使用分子生物学来克隆和测序组织特异性基因。这些基因的表达将在换羽期间被量化，并作为钙供应的函数。在所有真核细胞中，钙泵是钙稳态的基础。这项研究将勾勒出这种关键分子在过去6亿年的演变。