A GENETIC APPROACH TO VERTEBRATE NEURAL DEVELOPMENT

我的目标是了解神经组织的遗传控制 脊椎动物。我已经启动了一个新的方法来解决这个问题 视网膜作为中枢神经系统图案形成的模型：我们 将分离斑马鱼视网膜中的特定突变并使用表型 视网膜控制基因的分析和分子生物学研究 组织。 斑马鱼提供了一个发展极快移动的机会 探索发育基因的遗传系统。它的主要优势是 为遗传学开发孤雌生殖发育的能力。 我描述的程序将使将基因方法用于 大规模分离与特定基因相关的脊椎动物新基因 功能。将开发用于筛选数千名转基因生物的方法 插入突变和LacZ鉴定视网膜基因的品系 表情。突变体将被用于研究细胞间的相互作用。 参与组装视网膜和调节这些的分子 互动。

My goal is to understand the genetic control of neural organization in vertebrates. I have initiated a new approach to this problem using the retina as a model for patten formation in the central nervous system: we will isolate specific mutations in the zebrafish retina and use phenotype analysis and molecular biology to characterize genes controlling retinal organization. The zebrafish provides an opportunity to develop an extremely fast-moving genetic system to explore developmental genes. Its primary advantage is the ability to exploit parthenogenetic development for genetics. The procedures I describe will make it possible to use genetic methods on a large scale to isolate novel vertebrate genes involved in specific functions. Methods will be developed for screening thousands of transgenic lines to identify retinal genes by insertional mutation and by lacZ expression. Mutants will be used to study the cellular interactions involved in assembling a retina and the molecules that mediate these interactions.