A GENETIC APPROACH TO VERTEBRATE NEURAL DEVELOPMENT

脊椎动物神经发育的遗传方法

• 批准号: 3088896
• 负责人: Carl Fulwiler
• 金额: $ 9.58万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-08-01 至 1994-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3088896
• 关键词: alternatives to animals in research; cell cell interaction; developmental genetics; developmental neurobiology; fresh water environment; gene expression; gene mutation; genetic manipulation; genetic regulation; genetically modified animals; growth /development; molecular biology; retina; transfection; visual pathways; zebrafish

My goal is to understand the genetic control of neural organization in vertebrates. I have initiated a new approach to this problem using the retina as a model for patten formation in the central nervous system: we will isolate specific mutations in the zebrafish retina and use phenotype analysis and molecular biology to characterize genes controlling retinal organization. The zebrafish provides an opportunity to develop an extremely fast-moving genetic system to explore developmental genes. Its primary advantage is the ability to exploit parthenogenetic development for genetics. The procedures I describe will make it possible to use genetic methods on a large scale to isolate novel vertebrate genes involved in specific functions. Methods will be developed for screening thousands of transgenic lines to identify retinal genes by insertional mutation and by lacZ expression. Mutants will be used to study the cellular interactions involved in assembling a retina and the molecules that mediate these interactions.

我的目标是了解神经组织的遗传控制， 脊椎动物我已经开始了一个新的方法来解决这个问题， 视网膜作为中枢神经系统模式形成的模型：我们 将分离斑马鱼视网膜中的特定突变， 分析和分子生物学来表征控制视网膜的基因 organization. 斑马鱼提供了一个机会， 基因系统来探索发育基因。它的主要优点是 利用孤雌生殖发育进行遗传学研究的能力。 我所描述的程序将有可能使用遗传方法对一个 大规模分离新的脊椎动物基因， 功能协调发展的将开发用于筛选数千个转基因的方法。 通过插入突变和lacZ鉴定视网膜基因 表情突变体将用于研究细胞相互作用 参与了视网膜的组装以及调节这些分子的作用 交互.

项目成果

Retinal patterning in the zebrafish mutant cyclops.

斑马鱼突变独眼巨人的视网膜图案。

• 发表时间: 1997
• 期刊: The Journal of comparative neurology.
• 作者: Fulwiler,C;Schmitt,EA;Kim,JM;Dowling,JE
• 通讯作者: Dowling,JE