HEMOGLOBIN SYNTHESIS IN THE HUMAN ERYTHROCYTE

本提案研究部分的具体目标是 来确定人类中功能基因的相互作用 α珠蛋白基因簇。 I期研究将尝试 定义和优化实验系统，以研究 这些基因的表达，在第二阶段，这些研究将 扩展到处理基因控制的特定机制 包括：位置效应、转录干扰、3' 控制元件（转录终止信号和 增强子），以及一个基因转录的一般影响 基因簇中其他基因的功能。 结果 将与珠蛋白基因转换的机制有关， 基因控制的一般机制在密集的集群。 有计划的培训，以及具体的实验 有待探索系统旨在相互作用， 申请人具有未来独立工作的坚实背景。

The specific goals of the research component of this proposal are to define the interactions of the functional genes in the human alpha globin gene cluster. Phase I studies will attempt to define and optimize experimental systems in which to study the expression of these genes and in Phase II these studies will be extended to deal with specific mechanisms of gene control including; position effects, transcriptional interference, 3' control elements (transcriptional termination signals and enhancers), and the general impact of one genes transcription upon the function of other genes in the cluster. The results will be related to the mechanisms of globin gene switching and general mechanisms of gene control in tightly spaced clusters. The training which is planned, and the specific experimental system to be explored are designed to interact and provide that applicant with a firm background for future independent work.