STUDIES ON GENE EXPRESSION DURING GRANULOPOIESIS

本申请是一项研究培训补助金，允许申请人 在蛋白质化学、免疫化学和细胞方面有研究经验 生物学，同时扩展他以前在重组方面的训练， DNA方法，通过研究基因表达的控制 在粒细胞生成过程中。 研究计划分为两个阶段。 在第一阶段，申请人打算在弗兰克博士身上进行研究。 Ruddle的实验室对转铁蛋白受体基因的调节， 早幼粒细胞白血病细胞系。 DNA-蛋白质印迹法 最近在拉德尔博士的实验室开发的新技术将被用于 表征调节转铁蛋白转录的蛋白质 受体基因 第二阶段将在爱德华·本茨博士的实验室里度过 并将包括对基因调控的研究， 转铁蛋白受体基因在粒细胞生成成熟期的作用 例如诱导早幼粒细胞白血病细胞系， 终末成熟 调节这些基因转录的蛋白质 将被定性。 这些研究预计将给申请人 在分子生物学的所有领域的广泛培训，同时 对粒细胞生成的调节产生启发性的信息。

This application is for a research training grant to allow the applicant to gain research experience in protein chemistry, immunochemistry and cell biology and at the same time extend his previous training in recombinant DNA methods, by conducting research on the control of gene expression during granulopoiesis. The research program is divided into two phases. In Phase I the applicant intends to carry out research in Dr. Frank Ruddle's laboratory on the regulation of the transferrin receptor gene in a promyelocytic leukemia cell line. A DNA-protein blotting procedure recently developed in Dr. Ruddle's laboratory will be utilized to characterize proteins that regulate transcription of the transferrin receptor gene. Phase II will be spent in the laboratory of Dr. Edward Benz and will consist of studies on the regulation of genes other than the transferrin receptor gene during the maturation phase of granulopoiesis as exemplified by the induction of a promyelocytic leukemia cell line to terminal maturation. Proteins regulating the transcription of these genes will be characterized. These studies are expected to give the applicant broad training in all areas of molecular biology and at the same time produce illuminating information on the regulation of granulopoiesis.