CENTER FOR FAST KINETICS RESEARCH

自NIH首次资助以来的6年里，快速中心 Kinetics Research(CFKR)汇集了独特的技术组合 产生和分析快速瞬变的能力(次级到 稳定状态)。5 MV范德格拉夫加速器(VDG)产生电子脉冲 用于脉冲辐解，第二个VDG正在重建。脉冲式的 氮气激光器和可调谐染料激光器为闪光提供光子脉冲 光解，这种能力被扩展到亚纳秒区域 激光系统是一台条纹相机.瞬变的几种技术 提供了包括光发射和吸收光谱在内的检测， 传导性和极谱。停流快速混合与稳态 也有荧光分光光度法可用。所有系统都是计算机化的，因此 在实验过程中可以立即读取数据，从而实现快速分析 和改变实验条件，为研究人员节省了无数个小时 实验后数据约简。这些实验能力加在一起 在CFKR工作人员的亲自服务下，生物 以及全国各地的生物医学研究人员(目前为50人)。快速动能 技术在生物科学中具有广泛的适用性，包括 不同的研究领域，如化学辐射增敏机理 肿瘤的时间分辨荧光去偏振生物学研究 膜的结构和功能、视觉的光物理和光化学 色素、受辐射细胞中的O2效应以及许多其他因素。CFKR是 不断寻求开发新的快速动力学技术并应用现有的 涉及生物医学感兴趣的新问题；进行跨学科研究 与外部研究人员在前景看好的新领域合作；服务于更广泛的 主要由希望申请的生物科学家组成的用户群 快速动能技术到自己感兴趣的研究领域；快速训练 能够弥合快速发展的器械之间差距的运动学家 技术及其应应用于的生物问题，以及 公布这些努力的结果和进一步发展的潜力 这些方法在应用方面的进展。

During the 6 years since it was first funded by NIH, the Center for Fast Kinetics Research (CFKR) has assembled a unique combination of technical capabilities for the production and analysis of fast transients (sub-ns to steady-state). A 5 MV Van de Graaff accelerator (VdG) produces electron pulses for pulse radiolysis and a second VdG is under reconstruction. A pulsed nitrogen laser and a tunable dye laser provide photon pulses for flash photolysis, and this capability is extended into the sub-nanosecond region by a Nd:YAG laser system an streak camera. Several techniques for transient detection are provided, including optical emission and absorption spectroscopy, conductivity, and polarography. Stoppedflow fast mixing and steady-state spectrofluorimetry are also available. All systems are computerized so that immediate readout is available during the experiment, allowing quick analysis and change of experimental conditions and saving the researchers countless hours of post-experiment data reduction. These experimental capabilities, together with the personal services of the CFKR staff, are made available to biological and biomedical researchers (currently 50) throughout the nation. Fast kinetic techniques are of wide applicability in the biological sciences, encompassing such diverse research areas as the mechanism of chemical radiation sensitization of tumors, time-resoved fluorescence depolarization studies of biological membrane structure and function, the photophysics and photochemistry of visual pigments, the O2 effect in irradiated cells and many others. The CFKR is continually seeking to develop new fast kinetic techniques and to apply existing ones to new problems of biomedical interest; to undertake interdisciplinary collaboration with outside researchers in promising new fields; to serve a wider group of users composed primarily of biological scientists who wish to apply fast kinetic techniques to their areas of research interest; to train fast kineticists who can bridge the gap between rapidly advancing instrumentsal technology and the biological problems to which it should be applied, and to publize both the results of these endeavors and the potential for further advancement in the application of these methods.