PHYSICO-CHEMICAL STUDIES OF RADIATION EFFECTS IN CELLS

目的：描述导致该疾病的物理和化学事件的特征。 辐射损伤在细胞水平的表达。 方法：上述目标主要是通过使用 以巨大芽孢杆菌的芽胞为试验有机体。其他系统已经并将 例如噬菌体T7(过去使用)和转导DNA(目前在 使用)。X光和紫外光都被使用过。的脉冲辐解研究 快速动力学研究中心现在开始获得化学数据 相对于生物数据。 结果：提出了溶液中辐射增敏模型。 提出了涉及OH自由基和双氧水的方法。许多人的敏感化作用 包括铜、铁、钴、铂和银在内的金属离子已经被生物和 与这些生物学结果相关的化学研究目前正在进行中，以便 了解辐射敏化的机制。机制 涉及有机增感剂，如PNAP和双乙酰。 已有关于辐射对DNA和DNA转化的影响的结果 不同O2浓度的影响。清道夫和银的存在 建立了离子和铜对转化DNA的敏化作用。一个 重要的发现是在孢子中存在三种O2效应 停职。溶液中核苷酸和完整DNA的脉冲辐解研究 正在进行中。

OBJECTIVE: To characterize the physical and chemical events leading to the expression of radiation-induced damage at the cellular level. METHODS: The above objective has been approached mainly through the use of the spores of Bacillus megaterium as the test organism. Other systems have and will be used such as Phage T7 (used in the past) and transfoming DNA (currently in use). Both X-rays and U.V. have been used. Pulse radiolysis studies at the Center for Fast Kinetics Research are now beginning to obtain chemical data relative to biological data. RESULTS: A proposed model of radiation sensitization in solution has been presented involving the OH radical and H2O2. The sensitizing action of many metal ions including Cu, Fe, Co, Pt and Ag has been studied biologically and chemistry studies relative to these biological results are now underway so as to understand the mechanisms involved in radiation sensitization. Mechanisms involving organic sensitizers such as PNAP and diacetyl have been studied. Results have been obtained on the effects of radiation on transforming DNA and the effects of various O2 concentrations. OH scavengers and the presence of Ag ion and Cu on sensitization of transforming DNA have been established. A significant discovery is the existence of three O2 effects in spores in suspension. Pulse radiolysis studies on nucleotides and intact DNA in solution are in progress.