ROLE OF ALCOHOL IN THE DEVELOPMENT OF AIDS

酒精作为艾滋病毒感染的辅助因子的潜在作用， 流行病学研究表明， 研究，但酒精影响的实验证据是 缺乏，我们分离外周血单核细胞（PBM） 细胞从六个正常志愿者之前和之后40盎司。啤酒 (1.2 L）或其他酒精中的同等剂量乙醇 饮料. 这些细胞在体外暴露于HIV病毒。 感染后， 酒精摄入量作为评估的数目synctia形成（p 小于0.001）和PBM中释放的P24抗原水平 培养（p <0.001）。 未感染培养完成 同时表明，酒精摄入也降低了 淋巴细胞产生白细胞介素2（IL-2）的能力， 可溶性免疫反应抑制因子（SIRS）（p <0.05） 0.001），主要由辅助性和抑制性T细胞合成， 分别 我们的数据强烈表明， 酒精和通过性行为接触病毒或B 滥用药物时使用的受感染针头可能会增强传染性， 艾滋病。 以下具体目标并不相互排斥， 有助于进一步阐明潜在的细胞和 体液事件，导致随后的减少， 宿主对HIV的抵抗力。 1. 为了进一步阐明酒精诱导的 人外周血单个核细胞的易感程度 从正常人到艾滋病病毒感染者。 酒量 消耗量及其对免疫系统的不利影响的持续时间 系统将进行检查。 2. 为了阐明乙醇诱导的细胞凋亡的潜在机制， 在细胞和体液水平的免疫调节。 3. 寻找代理（例如，淋巴因子，单核因子，各种 增长因素等）这可能会中和或逆转 乙醇引起的不良反应。

The potential role of alcohol as a cofactor in HIV infection and development of AIDS has been suggested by epidemiologic studies, but experimental evidence for the effect of alcohol is lacking, we isolated the peripheral blood mononuclear (PBM) cells from six normal volunteers before and after 40 oz. of beer (1.2 L) or the equivalent dose of ethanol in other alcoholic beverages. These cells were exposed in vitro to HIV virus. There was a significant decrease of resistance to infection after alcohol ingestion as assessed by the number of synctia formed (p less than 0.001) and levels of P24 antigen released in PBM cultures (p less than 0.001). Noninfected cultures done simultaneously showed that alcohol ingestion also decreased the abiltity of lymphocytes to produce interleukin 2 (IL-2) and soluble immune response suppressor factor (SIRS) (p less than 0.001), synthesized preminiarly be helper and suppressor T cells, respectively. Our data strngly suggest that the concomitant use fo alcohol and exposure to the virus through sexual activity or b infected needles used in drug abuse may enhance infectivity by HIV. The Specific Aims below are not mutually exclusive and will contribute towards further elucidation of underlying cellular and humoral events which result in subsequent decrease in the resistance of host to HIV. 1. To further elucidate the alcohol-induced increase in the degree of susceptibility of human peripheral blood mononuclear cells from normal subjects to HIV infection. Amount of alcohol consumption and duration of its adverse effect on the immune system will be examined. 2. To elucidate the underlying mechanisms of ethanol induced immunomodulation at cellular and humoral levels. 3. To seek out agents (e.g., lymphokines, monokines, various growth factors etc.) which might neutralize or reverse the adverse effects induced by ethanol.