BIOSYNTHESIS OF BACTERIAL CELL WALL COMPONENTS

我们研究计划的主要目标是了解 分子水平上的细胞壁生物合成。合成、组装和 这种结构的放大是由一系列整合的 与膜相关的酶，由生物体仔细调节。我们的 研究将有助于了解这些酶、膜事件 在肽聚糖合成中，膜-壁的相互关系和膜 磷壁酸生物合成。此外，我们的目标是应用以下成果 我们对抗菌药物的设计进行了研究，以抑制细胞的组装 交联肽多糖。六个具体目标将为 即将到来的一年。(1)隔离墙在稳定组织方面的作用 参与处理初生的肽聚糖的酶。(2) 同时冻融的膜和壁的重建。 (3)冻融膜加成法生产初生肽聚糖 到墙上去。(4)青霉素和D-蛋氨酸对新生幼虫的作用机制 肽多糖的合成。(5)丹参监测的特定微环境 记者组在肽聚糖组装过程中。(6)机制和 脂磷壁酸的D-丙氨酸酰化反应：D-丙氨酰-亲脂性的作用 中间体和D-丙氨酸：膜受体连接酶。对……的调查 这些项目将有助于我们对细胞壁组装和 膜化学与这一过程有关的膜化学我们目前的目标将是 提供对以下方面发生的事件、法规和移位的见解 用于阐述这种细胞外结构的细菌膜，即细胞 墙。

The primary goal of our research program is directed towards an understanding of cell wall biosynthesis on the molecular level. The synthesis, assembly, and amplification of this structure is catalyzed by an integrated series of membrane-associated enzymes that are carefully regulated by the organism. Our research will contribute to an understanding of these enzymes, membrane events in peptidoglycan synthesis, membrane-wall interrelationships, and membrane teichoic acid biosynthesis. In addition, it is our goal to apply the results of our studies to the design of antibacterial agents that inhibit the assembly of cross-linked peptidoglycan. Six specific objectives will be undertaken for the coming year. (1) Role of the wall in stabilizing the organization of those enzymes that are involved in processing nascent peptidoglycan. (2) Reconstitution of membranes and walls that are simultaneously freeze-thawed. (3) Processing of nascent peptidoglycan by freeze-thawed membranes for additon to walls. (4) Mechanisms of penicillin and D-methionine actions on nascent peptidoglycan synthesis. (5) Specific microenvironments monitored by the dansyl reporter group during the course of peptidoglycan assembly. (6) Mechanism and D-alanine acylation of lipoteichoic acid: role of D-alanyl-lipophilic intermediates and D-alanine: membrane acceptor ligase. An investigation of these projects will contribute to our understanding of cell wall assembly and membrane chemistry that relates to this process. Our present objectives will provide insights into the events, regulations, and translocations that occur in the bacterial membrane for elaboration of this extracellular structure, the cell wall.