BIOSYNTHESIS OF BACTERIAL CELL WALL COMPONENTS

我们研究计划的主要目标是了解 细胞壁生物合成在分子水平上。 合成、组装和 这种结构的放大是由一系列整合的 由生物体仔细调节的膜相关酶。 我们 研究将有助于了解这些酶，膜事件 在肽聚糖合成、膜壁相互关系和膜 磷壁酸生物合成 此外，我们的目标是将 我们的研究设计的抗菌剂，抑制组装 交联肽聚糖。 将实现六个具体目标 来年 (1)隔离墙在稳定 参与处理新生肽聚糖的酶。 （二） 同时冻融的膜和壁的重建。 (3)用冻融膜处理新生肽聚糖 墙。 (4)青霉素和D-甲硫氨酸对新生血管的作用机制 肽聚糖合成。 (5)由丹磺酰监测的特定微环境 在肽聚糖组装过程中的报告基团。 (6)机制和 脂磷壁酸的D-丙氨酸酰化：D-丙氨酰-亲脂性的作用 中间体和D-丙氨酸：膜受体连接酶。 调查 这些项目将有助于我们理解细胞壁组装， 与此过程相关的膜化学。 我们目前的目标将 提供事件的见解，法规，和发生在 细胞膜是用来制造这种细胞外结构的， 墙

The primary goal of our research program is directed towards an understanding of cell wall biosynthesis on the molecular level. The synthesis, assembly, and amplification of this structure is catalyzed by an integrated series of membrane-associated enzymes that are carefully regulated by the organism. Our research will contribute to an understanding of these enzymes, membrane events in peptidoglycan synthesis, membrane-wall interrelationships, and membrane teichoic acid biosynthesis. In addition, it is our goal to apply the results of our studies to the design of antibacterial agents that inhibit the assembly of cross-linked peptidoglycan. Six specific objectives will be undertaken for the coming year. (1) Role of the wall in stabilizing the organization of those enzymes that are involved in processing nascent peptidoglycan. (2) Reconstitution of membranes and walls that are simultaneously freeze-thawed. (3) Processing of nascent peptidoglycan by freeze-thawed membranes for additon to walls. (4) Mechanisms of penicillin and D-methionine actions on nascent peptidoglycan synthesis. (5) Specific microenvironments monitored by the dansyl reporter group during the course of peptidoglycan assembly. (6) Mechanism and D-alanine acylation of lipoteichoic acid: role of D-alanyl-lipophilic intermediates and D-alanine: membrane acceptor ligase. An investigation of these projects will contribute to our understanding of cell wall assembly and membrane chemistry that relates to this process. Our present objectives will provide insights into the events, regulations, and translocations that occur in the bacterial membrane for elaboration of this extracellular structure, the cell wall.