CARILAGE MATRIX PROTEIN INTERACTIONS--CHANGES WITH AGE

软骨基质蛋白(Cmp)是大多数 软骨。已发表的研究提供了一些关于它的信息 组成、性质和分布。重组病毒的分离和测序 来自鸡和人的CP基因揭示了高度的 同源性，它们都具有广泛的序列，这是一个共同的 其他蛋白质的胶原蛋白结合区。来自Heinegdrd实验室的研究 我的研究表明，CMP也能与蛋白多糖结合。所以化学机械抛光可能会执行 通过与这两个主要机构的互动发挥重要的结构性作用 软骨细胞外基质的成分。 在本提案中，研究的目的是确认和进一步 描述软骨中cMP的相互作用。互动将是 使用体外结合法、化学交联法和 免疫组织学观察。单抗将被提出，并 以便于这项工作为特征的。 众所周知，化学机械抛光的量和不解性急剧增加。 随着年龄的增长。本提案的另一个目标是建立 这种不溶性的基础上，并确定化学机械抛光是否像许多其他 软骨的结构分子，变得越来越碎片化 年龄。 这项研究有望增加我们对正常结构的认识 软骨的组织以及随年龄增长而发生的主要变化。

The cartilage matrix protein (CMP) is a major constituent of most cartilages. Published studies have provided some information on its composition, properties and distribution. Isolation and sequencing of the gene for CMP from chick and human sources reveals a high degree of homology, and they both possess extensive sequences which are common to a collagen-binding region of other proteins. Work from Heinegdrd's lab and mine has demonstrated that CMP also binds proteoglycan. So CMP may perform an important structural role through interactions with these two major elements of cartilage extracellular matrix. In the present proposal, studies are designed to confirm and further characterize the interactions of CMP in cartilage. Interactions will be studied using in vitro binding methods, chemical cross-linking and by immunohistological observation. Monoclonal antibodies will, be raised and characterized to facilitate this work. It is known that the amount and insolubility of CMP increases dramatically with age. An additional goal of the present proposal is to establish the basis of this insolubility, and to determine whether CMP, like many other structural molecules of cartilage, becomes increasingly fragmented with age. This study is expected to add to our knowledge of the normal structural organization of cartilage and of major changes which occur with age.