ERYTHEMA MULTIFORME--A CLINICAL PATHOGENETIC STUDY

本研究的长期目标是了解 型多形红斑指定EM轻微。自从发现 大多数轻微EM病例与单纯疱疹有关 病毒[单纯疱疹病毒]，一种新的假设，皮肤病变的EM小发展 作为针对病毒的HSV特异性免疫应答的结果 在皮肤的这些部位已经发展。发现HSV 核酸可能存在于EM轻微愈合的皮肤病变中， 潜伏病毒状态的可能性在这项研究中， 病毒学技术，例如通过PCR扩增HSV特异性核酸， 聚合酶链反应（PCR）将用于检测重要 关于EM轻微发病机制的临床问题。这项研究将 重点关注EM皮肤部位内的病毒状态， 检测单纯疱疹病毒的潜伏期相关转录本[LATS]。 将建立皮肤细胞中单纯疱疹病毒潜伏期的模型。 通过限制性内切酶分析的DNA指纹分析等策略， 疱疹病毒分离物和通过血液和EM皮肤的PCR进行病毒分型， 就业。将采用长期病毒共培养策略以获得 从EM皮肤和血液样本中复制病毒。这些研究 旨在观察EM皮肤是否是神经元HSV潜伏期的部位， 研究宿主-病毒相互作用的模型。

The long term goal of this study is to understand the pathogenesis of the type of erythema multiforme designated EM minor. Since the discovery that the large majority of cases of EM minor are associated with herpes simplex virus [HSV], a novel hypothesis that the skin lesions of EM minor develop as a result of a HSV-specific immune response directed against virus within those sites in the skin has been developed. It was found that HSV nucleic acids may be present in healed skin lesions of EM minor, leading to the possibility of a latent virus state. In this study molecular virology techniques such as amplification of HSV specific nucleic acids by the polymerase chain reaction [PCR] will be used to examine important clinical questions regarding the pathogenesis of EM minor. The study will focus on the state of the virus within EM skin sites and we will attempt to detect latency associated transcripts [LATS] of herpes simplex virus. A model of herpes simplex virus latency in skin cells will be established. Strategies such as DNA fingerprinting by restriction enzyme analysis of herpes viral isolates and viral typing by PCR of blood and EM skin will be employed. Long-term viral co-culture strategies will be employed to obtain replicating virus from EM skin and from blood samples. These studies are designed to see if EM skin is a site of extraneuronal HSV latency and as a model for study of host-viral interactions.