STUDY OF ANTI-IDIOTYPIC ANTIBODIES

拟议研究的总体目标是扩展我们对 抗独特型抗体的特异性和抗体结合特性 抗体的 抗独特型抗体是网络的一部分， 通过识别抗体的独特型来调节免疫反应 和受体。 我们计划研究抗独特型抗体的结构 针对T15独特型形成，以剖析这种免疫应答。 T15是在抗磷酸胆碱反应中观察到的主要独特型 BALB/c小鼠。 我们将使用杂交瘤衍生的抗独特型小鼠 针对单克隆鼠骨髓瘤蛋白T15制备的抗体， 抗磷酸胆碱（PC）活性。 为了了解功能和 抗独特型抗体的相对重要性，并绘制出 T15分子上的独特型决定簇，几个 T15的抗原结合位点特异性和近位点特异性蛋白 分子进行比较。 抗抗体将进一步 其特征在于通过研究它们的结合的变化的影响， 离子强度、pH值、温度和 T15蛋白的PC结合位点中的精氨酸。 的结合亲和力 抗体分子及其Fab片段的量将使用 小区域凝胶过滤，应用我们开发的方法。

The overall goal of the proposed research is to extend our knowledge of the specificity and antibody binding characteristics of anti-idiotypic antibodies. Anti-idiotypic antibodies form part of the network that regulates the immune response, by recognizing the idiotype of antibodies and receptors. We plan to study the structure of anti-idiotype antibodies formed against the T15 idiotype in order to dissect this immune response. T15 is the major idiotype observed in the anti-phosphorylcholine response in BALB/c mice. We will work with hybridoma derived anti-idiotype murine antibodies prepared against a monoclonal murine myeloma protein, T15, with anti-phosphorylcholine (PC) activity. To understand the function and relative importance of the anti-idiotypic antibodies and to map out the idiotypic determinant on the T15 molecule, the binding affinity of several antigen binding site specific and near site specific proteins for the T15 molecule will be compared. The anti-antibodies will be further characterized by studying the effects on their binding of the variation of ionic strength, pH, temperature, and the chemical modification of an arginine in the PC binding site of the T15 protein. The binding affinities of the antibody molecules and their Fab fragment will be determined using small zone gel filtration, applying a method developed by us.