BASOPHIL HYPERSENSITIVITY AND IMMUNE HOST RESISTANCE

这些研究的目的是确定元素的作用 即刻超敏反应--过敏性疾病机制，如 嗜碱性粒细胞、肥大细胞和过敏性抗体(IgE和IgG1在 动物)，在对寄生虫的免疫抵抗反应中。 扁虱是动物和人类重要的体外寄生虫，也是 许多感染性病原体的媒介。对蚤的免疫抵抗力反应 伴随着非常大的嗜碱性粒细胞渗入。我们发现， 需要嗜碱性粒细胞来影响体外寄生虫的免疫排斥反应 嗜碱性粒细胞的渗透依赖于抗森林抗体IgG1 抗体和致敏细胞上。 在计划中的新实验中，我们将确定宿主Fc受体是否 与这些抗体介导免疫的作用机制有关的 皮肤嗜碱性细胞对扁虱的抵抗力反应。我们还将确定 如果IgE抗体也同样参与其中，我们将确定T细胞 细胞负责细胞介导的抵抗力。这些后一项研究 将通过在体外分离致敏细胞来完成，还 用抗T细胞的单抗进行体内治疗。这一机制 嗜碱性细胞对扁虱的抗药性将在#年进行调查 处理嗜碱性粒细胞衍生的介体的实验，如组胺， 花生四烯酸代谢的促凝剂和产物。 在随后的实验中，我们将调查新识别出的 抗原特异性T细胞因子参与嗜碱性粒细胞依赖免疫 豚鼠对体外寄生虫(蜱虫)的抗性反应。 这些研究应该进一步提高对猪免疫耐药的认识。 寄生虫，还应提供更多关于生物的信息 过敏性疾病发病机制的价值。

The purpose of these studies is to determine the role of elements of immediate hypersensitivity - allergic disease mechanisms, such as basophils, mast cells and anaphylactic antibodies (IgE and IgG1 in animals), in immune resistance responses to parasites. Ticks are important ectoparasites of animals and humans and are also vectors of many infectious agents. Immune resistance responses to ticks are accompanied by very large basophil infiltrates. We have found that basophils are required to effect this immune rejection of ectoparasites and that the infiltration of basophils is dependent on anti-tick IgG1 antibodies and on sensitized cells. In planned new experiments we will determine whether host Fc receptors are involved in the mechanism of action of these antibodies that mediate immune cutaneous basophil resistance responses to ticks. We will also determine if IgE antibodies are similarly involved and we will identify whether T cells are responsible for cell-mediated resistance. These latter studies will be accomplished by fractionation of sensitized cells in vitro and also by treatment in vivo with monoclonal anti-T cell antibodies. The mechanism of basophil dependent resistance to ticks will be investigated in experiments dealing with basophil derived mediators such as histamine, procoagulants and products of arachadonic acid metabolism. In subsequent experiments we will investigate whether newly recognized antigen specific T cell factors are involved in basophil-dependent immune resistance responses to ectoparasites (ticks) in guinea pigs. These studies should further advance knowledge of immune resistance to parasites, and should also provide more information about the biological value of allergic disease mechanisms.