IMMUNOLOGIC STUDIES IN ECTOTHERMIC VERTEBRATES

拟议研究的主要重点是更好地了解 细胞方面的免疫反应在变温动物。 广大 研究的目标有三个方面。 首先，建议测试 假设鱼有两类淋巴细胞可与T 以及哺乳动物和鸟类的B细胞。 测试这个假设将涉及 应用抗鲶鱼单克隆抗体的细胞分离技术 淋巴细胞表面成分结合淋巴细胞体外测定 功能协调发展的 第二个目标是检验低 鱼类的温度免疫抑制主要是由于 对辅助细胞生成的影响。 检验这一假设将 涉及温度对心脏的影响的体内和体外研究， 产生T依赖性和T非依赖性抗体分泌细胞 抗原 第三个目标是验证鱼类T细胞 具有与鱼B细胞不同的膜组成，因此， 适当的低温适应（等粘适应）， 在较低的温度下发生有丝分裂反应，如鱼B细胞。 验证这一假设将涉及对孤立的鲶鱼B和T的研究 细胞质膜和有丝分裂过程中发生的细胞事件 不同温度下的反应。 乐观地说，这些拟议中的研究 将产生关于系统发育的重要信息 免疫反应和激活的基本特征， 淋巴细胞的分化和相互作用。

The major emphasis of the proposed research is to better understand the cellular aspects of immune responses in ectothermic animals. The broad objectives of the research are threefold. Firstly it is proposed to test the hypothesis that fish have two classes of lymphocytes comparable to T and B cells of mammals and birds. Testing this hypothesis will involve cell separation techniques employing monoclonal antibodies to catfish lymphocyte surface components combined with in vitro assays of lymphocyte functions. The second objective is to test the hypothesis that low temperature immunosupression in fish results primarly from a preferential effect on the generation of helper cells. Testing this hypothesis will involve in vivo and in vitro studies of temperature effects on the generation of antibody secreting cells to T-dependent an T-independent antigens. The third objective is to test the hypothesis that fish T cells have a different membrane composition than fish B cells and hence, without appropriate low temperature acclimation (homeoviscous adaptation), will not undergo mitogenic responses at lower temperatures as do fish B cells. Testing this hypothesis will involve studies on isolated catfish B and T cell plasma membranes and on cellular events occurring during mitogenic responses at various temperatures. Optimistically, these proposed studies will result in important information regarding the phylogenetic development of immune responses and the fundamental features of the activation, differentiation and interaction of lymphocytes.