NON-HLA HUMAN GRANULOCYTE, MONOCYTE ENDOTHELIAL ANTIGENS

这项研究的具体目的是检测发育性和 Non-T、Non-B上功能不同的多态和单态抗原 单核细胞、粒细胞和血管内皮细胞；使用 与这些抗原反应的抗体分离出表型标记的 这些细胞的子集；并确定细胞的功能特征 这些子集。将特别关注符合以下条件的异体抗血清 似乎识别具有特殊功能的细胞上的多态抗原 关于他们检测到的抗原的遗传，临床 这些抗体出现的环境和组织 决定因素的分布。这些研究可能会有相当大的影响 有关某些疾病的致病机制及 移植生物学。将使用单克隆抗体来识别 单态抗原的组织特异性和个体发育及分离 携带这些抗原的细胞，用于体外功能研究。我们有 产生了一组15种检测粒细胞的单抗， 非T、NO-B单核细胞和内皮细胞抗原。有了这个 小组，细胞标记物研究将在移植前后进行 以连续的时间间隔分析 淋巴细胞、单核细胞、自然杀伤细胞和树突状细胞 外周血、骨髓等组织。这些研究将 影响我们关于使用单克隆抗体的决定 预防和/或治疗各种疾病和移植。 这些抗体也将被用来检测患有 免疫缺陷疾病和人口老龄化。

The specific aims of this research are to detect developmentally and functionally distinct polymorphic and monomorphic antigens on non-T, non-B mononuclear cells, granulocytes and vascular endothelial cells; to use the antibodies reacting with these antigens to isolate phenotypically marked subsets of these cells; and to determine functional characteristics of these subsets. Particular attention will be paid to alloantisera that appear to identify polymorphic antigens on cells with specialized functions with respect to the inheritance of the antigens they detect, the clinical circumstances in which these antibodies have arisen and the tissue distribution of the determinants. These studies may shed considerable light on the pathogenetic mechanisms involved in certain diseases and transplantation biology. Monoclonal antibodies will be used to identify the tissue specificity and ontogeny of monomorphic antigens and to isolate cells bearing these antigens for in vitro functional studies. We have produced a panel of 15 monoclonal antibodies that detect granulocyte, non-T, no-B mononuclear cells and endothelial cell antigens. With this panel, cell marker studies will be carried out pre and post transplantation at sequential intervals assaying the appearance and frequency of lymphocytes, monocytes, natural killer cells and dendritic cells in the peripheral blood, bone marrow and other tissues. These studies will influence our decisions regarding the use of monoclonal antibodies in the prophylaxis and/or treatment of various diseases and transplantation. These antibodies will also be used to examine individuals with immunodeficiency disorders and aging populations.