LATERAL AND ROTATIONAL DYNAMICS IN BIOLOGICAL MEMBRANES

几种仪器技术将用于研究横向和 参与淋巴细胞刺激的膜分子的旋转运动 抗原和促性腺激素激活黄体细胞。 荧光 光漂白恢复（FPR）和图像增强视频显微镜（IIVM） 检查分子横向运动，同时偏振荧光耗尽 (PFD)测量蛋白质旋转松弛。 人工抗原修饰B淋巴细胞的研究 受体表现出对特异性 抗原 这些细胞的横向和旋转迁移率 抗原、抗原受体、整合膜蛋白和脂质探针 将被衡量。 结果将与自然界的数据进行比较。 抗原特异性细胞，以更好地了解表面的作用 免疫球蛋白在B细胞活化中作用。 B的膜动态要求 将T依赖性抗原呈递给T辅助细胞的细胞将是 研究了 B细胞Ia和辅助细胞蛋白水解的作用 这些研究应阐明抗原的处理。 数学 抗原与细胞表面受体相互作用的模型将得到扩展 他们的预测与实验数据进行了检验。 各种促性腺激素如促黄体激素（LH）和人促性腺激素的结合 绒毛膜促性腺激素与黄体细胞上LH受体的结合将通过以下方法进行检查： FPR和PFD。 这将显示受体复合物的迁移率 与孕酮反应的持续时间有关。 初始 分布、代谢诱导的再分布和内化位点 将通过电子显微镜和IIVM寻找LH受体，以评估 受体再分布和黄体细胞激活之间的联系。 LH受体的迁移率将通过交联人为限制， 测试受体动力学的变化是否积极影响黄体细胞 对促性腺激素的类固醇生成反应。 将更新和扩大FPR、PFD和IIVM的仪器设施。

Several instrumental techniques will be used to study lateral and rotational motions of membrane molecules involved in lymphocyte stimulation by antigens and in luteal cell activation by gonadotropins. Fluorescence photobleaching recovery (FPR) and image-intensified video microscopy (IIVM) examine molecular lateral motions while polarized fluorescence depletion (PFD) measures protein rotational relaxation. B lymphocytes decorated by a new technique with artificial antigen receptors exhibit polyclonal differentiative responses to specific antigens. The lateral and rotational mobilities on these cells of bound antigens, antigen receptors, integral membrane proteins and lipid probes will be measured. Results will be compared with data on natural antigen-specific cells to better understand the role of surface immunoglobulin in B cell activation. Membrane dynamic requirements for B cell presentation of T-dependent antigen to T helper cells will be investigated. The role of B cell Ia and accessory cell proteolytic processing of antigen should be clarified by these studies. Mathematical models for antigen interaction with cell surface receptors will be extended and their predictions tested against experimental data. Binding of various gonadotropins such as luteinizing hormone (LH) and human chorionic gonadotropin to LH receptors on luteal cells will be examined by FPR and PFD. This will show how mobilities of hormone-receptor complexes are related to the duration of progesterone responses. The initial distribution, hormone-induced redistribution, and sites of internalization of LH receptors will be sought by electron microscopy and by IIVM to assess the connection between receptor redistribution and luteal cell activation. LH receptor mobility will be artificially restricted by crosslinking to test whether changes in receptor dynamics actively affect luteal cell steroidogenic responses to gonadotropins. Instrumental facilities for FPR, PFD, and IIVM will be updated and expanded.