CELL BIOLOGY OF IMMUNE INTERACTIONS

其目的是详细了解分子事件， 是由淋巴细胞识别外来抗原触发的， 产生适当的免疫反应。 的结果予以 这些研究将增强我们对细胞的理解， 免疫系统可以处理病毒感染，有些是病毒引起 恶性肿瘤、器官移植和入侵微生物。 拟议研究的具体目标是： 1.为了研究辅助分子L3 T4、Lyt-2和LFA-1在 T细胞与其靶细胞的相互作用。 2.为了鉴定在抗原识别后， 参与跨膜信号的传递。 3.制备新的单克隆抗体，以鉴定新的 细胞骨架蛋白和膜蛋白， 效应器-靶接触区域。 4.目的：探讨酪氨酸激酶在T细胞和B细胞活化中的作用 活性，并且如果是，则鉴定磷酸化蛋白质。 5.为了确定抗原激活B细胞是否诱导细胞内 类似于T细胞激活过程中诱导的事件。 6.定位B细胞受体与特异性 辅助性T细胞

The objective is to gain a detailed insight into the molecular events that are triggered by lymphocyte-recognition of foreign antigens, which lead to the generation of the proper immune responses. The results of these studies will enhance our understanding of the means by which cells of the immune system can deal with viral infections, some virally-induced malignancies organ transplantations and invading microorganisms. The specific aims of the proposed research are: 1. To study the role of accessory molecules like L3T4, Lyt-2, and LFA-1 in the interactions of T cells with their targets. 2. To identify membrane proteins that, following antigen-recognition, are involved in transmitting transmembrane signals. 3. To prepare new monoclonal antibodies in order to identify new cytoskeletal proteins and membrane proteins which become segregated into the effector-target contact area. 4. To find if T cell and B cell activation involves tyrosine kinase activity, and if so, to identify the phosphorylated proteins. 5. To determine if B cell activation by antigen induces intracellular events like the ones induced during T cell activation. 6. To localize B cell receptors during their interaction with specific T-helper cells.