IMMUNOGENETICS OF TRICHINELLA SPIRALIS INTHE MOUSE

已知两种H-2基因Ts-1和Ts-2影响免疫反应 抗犬弓老鼠体内的螺旋体 有证据表明，T细胞 需要相互作用来产生有效的免疫应答，并且 易感小鼠表达Ts-2相关的缺陷，在一个相互作用的 T细胞群体。 免疫遗传学实验在遗传特征 H-2同源系小鼠将确定T-T细胞是否 有效反应需要相互作用，表征细胞 参与，并确定表达Ts-2相关的细胞群， 缺损 为了实现这些目标，选择的淋巴结细胞群 将被注射到具有遗传特征的T细胞缺陷或正常的 小鼠 这些小鼠将被T.螺旋及其水平 电阻测量 T细胞缺陷的细胞接受者将 胸腺切除，辐射，并用同基因骨髓重建。 T.用于转移的螺旋体训练的T细胞将来自辐射的， 胸腺细胞重建小鼠或感染T. 螺旋形的 T.螺旋体培养的细胞将被注射到接受者体内 单独或与来自正常人的选定细胞群组合 小鼠 表达的Thy-1和Ly抗原特异性单克隆抗体 T细胞上表达的Ia抗原和B细胞、巨噬细胞和 T细胞的亚群将被用于仔细定义组成 细胞被转移。 这些体内实验的结果将是 通过研究细胞间的相互作用来证实， 最大体外增殖反应，高度纯化，保护 诱导T.螺旋体抗原 为补充上述研究， 进一步探讨T细胞和抗体在免疫中的作用 对T.螺旋，我们将测试在体内注射的效果， 用克隆的T细胞和特异于 T.螺旋形的 寄生虫诱导的作用 免疫抑制同样通过给小鼠注射 制备T.螺旋体特异性I-J+抑制因子 抑制性T细胞杂交瘤。 单克隆抗体、T细胞克隆和 抑制因子也将用于纯化和表征相关T. 螺旋体抗原

Two H-2 genes, Ts-1 and Ts-2, are known to influence the immune response against T. spiralis in the mouse. Evidence suggests that T-T cell interaction is required to generate an efficient immune response, and that susceptible mice express a Ts-2 associated defect in one of the interacting T cell populations. Immunogenetic experiments in genetically characterized H-2 congenic strains of mice will establish whether or not T-T cell interaction is required for an efficient response, characterize the cells involved, and identify the cell population expressing the Ts-2-associated defect. To accomplish these aims, selected populations of lymph node cells will be injected into genetically characterized, T cell deficient or normal mice. These mice will then be infected with T. spiralis and their levels of resistance measured. T cell deficient recipients of cells will be thymectomized, irradiated, and reconstituted with syngeneic bone marrow. T. spiralis-educated T cells used for transfer will be from irradiated, thymocyte reconstituted mice or from normal mice infected with T. spiralis. T. spiralis-educated cells will be injected into recipients either alone or in combination with selected cell populations from normal mice. Monoclonal antibody specific for the Thy-1 and Ly antigens expressed on T cells and for the Ia antigens expressed on B cells, macrophages and subpopulations of T cells will be used to carefully define the composition of cells being transferred. Results of these in vivo experiments will be confirmed by studying the cell-cell interactions required to generate a maximal in vitro proliferation response to highly purified, protection inducing T. spiralis antigens. To complement the above studies and to further explore the role of T cells and antibody in effecting the immune response against T. spiralis, we will test the in vivo effect of injecting mice with cloned T cells and with monoclonal antibody specific for protection inducing antigens of T. spiralis. The role of parasite-induced immunosuppression will likewise be explored by injecting mice with preparations of T. spiralis-specific I-J+ suppressor factors secreted by suppressor T cell hybridomas. Monoclonal antibody, T cell clones and suppressor factors will also be used to purify and characterize relevant T. spiralis antigens.