STUDIES ON RECOMBINATION IN THE MULTIPARTITE RNA VIRUSES

我们研究项目的总体目标是了解病毒编码的 信息决定传染性、宿主范围和其他相互作用， mRNA病毒与其宿主。 我们特别感兴趣的是 基因组RNA之间重组过程的分子机制 为这些病毒提供了一些特殊的手段， 快速进化 在这个项目中，我们将有助于了解重组 多体mRNA病毒中的突起。 我们将仔细研究三个 相关模式溴病毒：雀麦花叶病毒、豇豆褪绿斑驳病毒 病毒和蚕豆斑驳病毒。通过使用各种溴病毒RNA突变体， 我们将研究整个植物和植物原生质体中的重组。 各种溴病毒RNA组分之间的同源重组将是可能的。 使用编码区和非编码区的感染性突变进行研究。 为了研究序列和结构因素， 重组，重组位点将定位在溴病毒上， RNA基因组和这些位点周围的序列将被表征。 将进行实验以阐明RNA合成的哪个阶段 发生重组。 这项工作的主要重要性在于它对基本的 病毒学和生物学;结果直接适用于其他RNA 感染动植物的病毒。

The overall goal of our research project is to understand how virus-encoded information determines infectivity, host range and other interactions of mRNA viruses with their hosts. We are particularly interested in the molecular mechanisms by which recombination processes between genomic RNAs provide these viruses with some special means for their flexibility and rapid evolution. In this project we will contribute to the understanding of recombination processes in multipartite mRNA viruses. We will study three closely related model bromoviruses: brome mosaic virus, cowpea chlorotic mottle virus and broad bean mottle virus. By using various bromoviral RNA mutants, we will investigate recombination in whole plants and in plant protoplasts. Homologous recombination between various bromoviral RNA components will be studied using infectious mutations in both coding and noncoding regions. To investigate sequence and structural factors which determine recombination, the sites of recombination will be localized over bromoviral RNA genome and sequences around these sites will be characterized. Experiments will be undertaken to elucidate at what stage of RNA synthesis the recombination occurs. The primary importance of this work lies in its impact on fundamental virology and biology; the results are directly applicable for other RNA viruses infecting both animals and plants.