BACTERIAL COLONIZATION OF SURGICAL BIOMATERIALS

这一竞争性续订应用程序建议定义交互 在细菌细胞表面和生物材料底物之间。葡萄球菌 表皮葡萄球菌和金黄色葡萄球菌是聚合物中的主要病原体。 和基于金属合金的生物材料感染。从细菌到- 生物材料的相互作用是由囊外多糖介导的， 细菌受体、宿主衍生的条件膜和由物理 以及生物材料表面的化学性质。细菌的作用 囊外多糖(粘液)以及两种有意义的 调理膜的成分(纤维粘连蛋白和胶原) 某些细菌菌株已经确定了受体，将进行检查。 对生物材料的初级(初始)粘附率，而不是定植 金黄色葡萄球菌和胞外多糖的(原位生长) 将对非胞外多糖产生菌表皮葡萄球菌进行评估 作为后续目标的参考值。粘液在初选中的作用 粘附性将通过抗体抑制研究来确定和验证。 金黄色葡萄球菌已被证明具有胶原和纤维连接蛋白受体。 宿主衍生的条件膜部分由纤维连接蛋白和 胶原蛋白。调理膜的单一成分的作用可以部分地 通过对细菌赖以生存的蛋白质分子的研究来定义 已知有感受器。表面黏附与缓蚀研究 胶原蛋白和纤维连接蛋白的调节作用将决定这些 蛋白质及其相应的特定细菌受体。学位 这些受体在初始黏附中的作用将与之进行对比 具有预粘附性，用于生产煤泥和生产非煤泥 菌株。 本次调查的目的是：1)确定比较初级 金黄色葡萄球菌和表皮葡萄球菌对聚氨酯的黏附结合率， 超高相对分子质量聚乙烯、聚甲基丙烯酸甲酯、钛 合金(Ti6A14V)和二氧化硅(型号，无缺陷表面)，2) 表皮葡萄球菌胞外多糖在原代培养中的作用 生物材料的粘附性；胞外多糖抗体将用于 竞争抑制实验，以及3)测定结合率 金黄色葡萄球菌和表皮葡萄球菌对目标1中已被 用纤维连接蛋白或胶原包被；表位竞争抑制研究 将包括在内以确定所涉及的特定表位，并 确定沙门氏菌是否产生粘液。 表皮细胞具有纤维连接蛋白或胶原蛋白的功能性受体。

This competing renewal application proposes to define the interactions between bacterial cell surfaces and biomaterial substrata. Staphylococcus epidermidis and Staphylococcus aureus are principal pathogens in polymer- and metal alloy-based biomaterial infections respectively. Bacteria-to- biomaterial interactions are mediated by extracapsular polysaccharides, bacterial receptors, host derived conditioning films and by the physical and chemical nature of the biomaterial surface. The role of bacterial extracapsular polysaccharide (slime) as well as that of two significant components of conditioning films (fibronectin and collagen) for which certain strains of bacteria have defined receptors will be examined. Primary (initial) adhesion rates to biomaterials, rather than colonization (in situ growth) of S. aureus and exopolysaccharide- and nonexopolysaccharide-producing strains of S. epidermidis will be evaluated as reference values for subsequent aims. The role of slime in primary adhesion will be determined and validated by antibody inhibition studies. S. aureus has been shown to possess receptors for collagen and fibronectin. Host derived conditioning films are in part composed of fibronectin and collagen. The role of single components of conditioning films may in part be defined by studies of those protein molecules for which bacteria are known to have receptors. Adhesion and inhibition studies of surfaces conditioned with collagen and fibronectin will define the role of these proteins and their corresponding specific bacterial receptors. The degree to which those receptors function in initial adhesion will be contrasted with preliminary adhesion for slime producing and non-slime producing strains. The aims of this investigation are: 1) determine the comparative primary adhesion binding rates of S. aureus and S. epidermidis for polyurethane, ultra high molecular weight polyethylene, polymethylmethacrylate, titanium alloy (Ti6A14V) and silicon dioxide (model, defect-free surface), 2) determine the role of the exopolysaccharide of S. epidermidis in primary adhesion to biomaterials; exopolysaccharide antibodies will be used in competitive inhibition experiments, and 3) determine the binding rates of S. aureus and S. epidermidis for the biomaterials in Aim 1 which have been coated with fibronectin or collagen; epitope competitive inhibition studies will be included to determine the specific epitopes involved and to determine whether slime producing or non-slime producing strains of S. epidermidis have functional receptors for fibronectin or collagen.