BACTERIAL COLONIZATION OF SURGICAL BIOMATERIALS

此竞争性更新应用程序建议定义交互 细菌细胞表面和生物材料基质之间的关系。 葡萄球菌 表皮葡萄球菌和金黄色葡萄球菌是聚合物- 和基于金属合金的生物材料感染。 细菌对 生物材料相互作用由囊外多糖介导， 细菌受体，宿主衍生的调理膜和物理 和生物材料表面的化学性质。 细菌的作用 囊外多糖（粘液）以及两种重要的 调理膜的组分（纤连蛋白和胶原蛋白）， 将检测具有确定受体的某些细菌菌株。 与生物材料的原发性（初始）粘附率，而非定植 (in原位生长）。金黄色葡萄球菌和胞外多糖-和 非胞外多糖产生菌S.将对表皮进行评价 作为后续目标的参考值。 粘液在原发性肝癌中的作用 粘附将通过抗体抑制研究来确定和验证。 S.金黄色葡萄球菌已显示具有胶原蛋白和纤连蛋白的受体。 宿主衍生的调节膜部分由纤连蛋白和纤维蛋白酶组成。 胶原 调理膜的单一组分的作用可能部分地 通过对细菌所依赖的蛋白质分子的研究来定义。 已知有受体。 表面的粘附和抑制研究 与胶原蛋白和纤连蛋白条件将定义这些作用 蛋白质及其相应的特异性细菌受体。 程度 这些受体在初始粘附中的功能将被对比 带预粘的产粘和不产粘 菌株 本研究的目的是：1）确定比较小学 S.金黄色葡萄球菌和聚氨酯的表皮， 超高分子量聚乙烯、聚甲基丙烯酸甲酯、钛 合金（Ti6 A14 V）和二氧化硅（模型，无缺陷表面），2） 确定S.原发性表皮 粘附生物材料;外泌多糖抗体将用于 竞争性抑制实验，和3）确定的结合率 S.金黄色葡萄球菌和目标1中的生物材料的表皮， 用纤连蛋白或胶原包被;表位竞争性抑制研究 以确定所涉及的特异性表位， 确定S. 表皮具有纤连蛋白或胶原的功能性受体。

This competing renewal application proposes to define the interactions between bacterial cell surfaces and biomaterial substrata. Staphylococcus epidermidis and Staphylococcus aureus are principal pathogens in polymer- and metal alloy-based biomaterial infections respectively. Bacteria-to- biomaterial interactions are mediated by extracapsular polysaccharides, bacterial receptors, host derived conditioning films and by the physical and chemical nature of the biomaterial surface. The role of bacterial extracapsular polysaccharide (slime) as well as that of two significant components of conditioning films (fibronectin and collagen) for which certain strains of bacteria have defined receptors will be examined. Primary (initial) adhesion rates to biomaterials, rather than colonization (in situ growth) of S. aureus and exopolysaccharide- and nonexopolysaccharide-producing strains of S. epidermidis will be evaluated as reference values for subsequent aims. The role of slime in primary adhesion will be determined and validated by antibody inhibition studies. S. aureus has been shown to possess receptors for collagen and fibronectin. Host derived conditioning films are in part composed of fibronectin and collagen. The role of single components of conditioning films may in part be defined by studies of those protein molecules for which bacteria are known to have receptors. Adhesion and inhibition studies of surfaces conditioned with collagen and fibronectin will define the role of these proteins and their corresponding specific bacterial receptors. The degree to which those receptors function in initial adhesion will be contrasted with preliminary adhesion for slime producing and non-slime producing strains. The aims of this investigation are: 1) determine the comparative primary adhesion binding rates of S. aureus and S. epidermidis for polyurethane, ultra high molecular weight polyethylene, polymethylmethacrylate, titanium alloy (Ti6A14V) and silicon dioxide (model, defect-free surface), 2) determine the role of the exopolysaccharide of S. epidermidis in primary adhesion to biomaterials; exopolysaccharide antibodies will be used in competitive inhibition experiments, and 3) determine the binding rates of S. aureus and S. epidermidis for the biomaterials in Aim 1 which have been coated with fibronectin or collagen; epitope competitive inhibition studies will be included to determine the specific epitopes involved and to determine whether slime producing or non-slime producing strains of S. epidermidis have functional receptors for fibronectin or collagen.