PATHOGENICITY OF HIV ISOLATES--ROLE IN FETAL TRANSMISSON

这些研究试图了解目前存在的艾滋病毒变种的作用。 感染、妊娠妇女的免疫功能及其免疫应答 艾滋病毒从母亲传给胎儿的过程。我们假定 当针对变种的关键免疫反应发生时，可能会发生传播 未出现隔离或丢失。我们将分离出生物克隆 从受感染的母亲和婴儿那里获得艾滋病毒，并检查他们的生长速度和 合胞体形成能力，部分使用了一种新的测定方法，它可以量化 感染细胞对未感染的CD4阳性细胞的杀伤能力 合胞体类型的交互不需要高效的 在目标细胞中建立感染。 此外，我们还将研究来自感染者的CD8细胞的作用 限制不同的艾滋病毒生物克隆从给定的 作为对自体艾滋病毒的细胞免疫的一个例子，以及作为一个 筛选出生物变异的潜在方法。我们会比较一下 不同合胞体形成株gp120与CD4的结合 能力，使用细胞结合和酶联分析，我们有 发展起来的。为了更多地了解HIV对CD4的免疫反应 抗原，我们将研究来自 受感染的母亲和婴儿到一组艾滋病毒重组蛋白和 代表gp120特定区域的合成肽。 从母亲体内分离的HIV生物克隆的特征 婴儿和他们的免疫反应将被分析为他们的 与艾滋病毒从母亲传播到胎儿的关系。

These investigations seek to understand the role of variants of HIV present in infected, pregnant women and the immune responses to them in governing the transmission of HIV from mother to fetus. We postulate that transmission may occur when a critical immune response against a variant isolate does not occur or is lost. We will isolate biological clones of HIV from infected mothers and infants, and examine their growth rate and syncytia-forming capacity using, in part, a new assay that quantitates the ability of infected cells to kill noninfected CD4 positive cells by a syncytia type of interaction that does not require that a productive infection be established in the target cell. In addition we will study the role of CD8 cells from infected individuals to limit the outgrowth of different biological clones of HIV from a given patient, as an example of cellular immunity to autologous HIV, and as a potential method for selecting out biological variants. We will compare the binding to CD4 of gp120 from isolates of different syncytia-forming capacities, using both cell binding and an enzyme-linked assay we have developed. In order to learn more about CD4 immune responses to HIV antigens, we will study the proliferative responses of lymphocytes from infected mothers and infants to a panel of HIV recombinant proteins and synthetic peptides representing selected regions of gp120. The characteristics of the biological clones of HIV isolated from mothers and infants and the immune responses to them will be analyzed as to their association with the transmission of HIV from mother to fetus.