FACTORS INFLUENCING SURVIVAL OF VASCULARIZED BONE GRAFTS

这项申请的具体目的是继续调查 影响带血管蒂骨移植原理的因素。 目前可用的带血管蒂骨移植用于重建大块骨 骨广泛切除后受损床的节段性骨丢失 恶性骨肿瘤、创伤性骨缺损和感染性骨不连 由于结构上的确认，具有显著的局限性 移植物、供体部位发病率。这项提议的中心目标是 是利用生物合成成分(羟基磷灰石陶瓷模具)， 骨诱导原理与成骨细胞与成骨细胞 血管化蒂骨形成“模塑的血管化成骨”。 该方案的第二个目标是分离骨髓基质。 骨祖细胞，确定合适的程度 体内最大限度地促进成骨的分化，并将其扩展到 在模塑成骨中使用之前确定的体外群体。 已经设计了一些研究来分离具有特征性的成骨前体细胞 从兔骨髓中提取细胞，并利用扩散箱 确定与基质宿主细胞的适当接触程度，并 测定脱钙骨基质和生长因子的作用 异位骨再生的研究。这种带血管的模塑骨移植 将被用作岛状移植物或自由移植和愈合 这些移植物的材料特性将被检查并与 传统的骨移植。

The specific aims of this application are to continue the investigation of factors influencing the principles of vascularized bone grafts. Currently available vascularized bone grafts used to reconstruct massive segmental bone loss in compromised beds following wide resections of malignant bone tumors, traumatic bony defects, and infected non-unions have significant limitations resulting from structural confirmation of the graft, and donor site morbidity. The central goal of this proposal is to utilize biosynthetic components (hydroxyapatite ceramic mold), osteoinductive principles and osteoprogenitor cells coupled with a vascularized pedicle to produce "a molded vascularized osteogenesis". The second goal of this proposal is to isolate bone marrow stromal osteoprogenitor cells, determine the appropriate degree of differentiation that maximizes osteogenesis in vivo and expand this to defined populations ex vivo prior to utilization in molded osteogenesis. Studies have been designed to isolate the characterize osteoprogenitor cells from rabbit marrow in vitro and utilize diffusion chambers to determine the appropriate level of contact with matrix host cells and to determine the effects of demineralized bone matrix and of growth factors on heterotopic bone regeneration. This vascularized molded bone graft will be used as an island graft or a free transfer and the healing and material properties of these grafts will be examined and compared to conventional bone grafts.