ACTION OF HORMONES ON PROTEIN AND RNA METABOLISM

探讨雄激素诱导雄性化的机制， 在胚胎发育过程中的男性泌尿生殖道， 青春期的时间，并提供洞察的发病机制异常性 为了开发，计划进行各种实验。 其中包括调查 在培养的皮肤成纤维细胞中雄激素受体的分子缺陷 与男性相关的雄激素抵抗状态 假两性畸形，类固醇5 α-还原酶发病机制的研究 缺乏和靶组织中5 α-还原酶活性的调节， 双氢睾酮形成在雄激素分泌中的作用 使用5 α-还原酶活性抑制剂的生理学， 胚胎组织中雄激素受体机制的研究 性腺的内分泌分化，染色体性别最终 移植到表型性别，和遗传和酶的表征， 芳香化酶的反应，雄激素转化为雌激素， 外周组织，使用来自正常和母鸡的组织和成纤维细胞 羽毛的鸡和具有各种病理状态的患者。 因此，一个多方面的方法预计提供深入了解雄激素 代谢和行动在正常和进入pethophysiology正常 性发展也是如此。

To explore the mechanisms by which androgens act to induce masculinization of the male urogenital tract during embryogenesis and to virilize the male at the time of puberty and to provide insight into the pathogenesis of abnormal sexual development, a variety of experiments are planned. These include investigation in cultured skin fibroblasts of the molecular defects in the androgen receptor responsible for androgen resistance states associated with male pseudohermaphroditism, study of the pathogenesis of steroid 5 alpha-reductase deficiency and of the regulation of 5 alpha-reductase activity in target tissue, investigation of the role of dihydrotestosterone formation in androgen physiology using inhibitors of 5 alpha-reductase activity, characterization of the androgen receptor machinery in embryonic tissue, investigation of the endocrine differentiation of gonads by which chromosomal sex is ultimately transplanted into phenotypic sex, and a genetic and enzymatic characterization of the aromatase reaction by which androgens are converted to estrogens in peripheral tissues, using tissues and fibroblasts from normal and henny feathered chickens and from patients with a variety of pathological states. Thus, a multifaceted approach is projected to provide insight into androgen metabolism and action in the normal and into the pethophysiology of normal sexual development as well.