RENAL AMMONIA GENESIS: 15NH3 STUDIES WITH GC-MS

该项目建议在体外调查尿液来源。 氨氮。特别令人感兴趣的是氨基 谷氨酰胺以外的酸是肾氨的重要氮源， 取决于酸碱状态。PH值对氨生成的影响 谷氨酰胺的氨基或α氮基也是 学习。重点介绍了嘌呤核苷酸各自的作用。 作为代谢途径的循环和谷氨酸脱氢酶反应 肾缺氨。氨化作用与细菌生长发育的关系 α-酮戊二酸的代谢，特别是这种有机酸的作用 刺激谷氨酸生成氨的作用是另一个 调查重点。 实验将在大鼠肾皮质切片、离体肾 肾小管、肾线粒体和孤立的灌流肾。 该项目的一个独特特点是使用15N作为示踪剂 肾氮代谢与气相色谱-质谱仪的利用 用分光光度法定量测定该组织中的15N水平。数据是这样 将会对提高我们对 肾脏氮代谢。

This project proposes to investigate in vitro the sources of urinary ammonia nitrogen. Of particular interest is the possibility that amino acids other than glutamine are important nitrogen donors to renal ammonia, depending upon acid-base status. The influence of pH on ammonia formation from either the amide or alpha nitrogens of glutamine is also a target of study. Emphasis is placed on the respective roles of the purine nucleotide cycle and the glutamate dehydrogenase reactions as metabolic pathways of renal ammoniagenesis. The relationship between ammoniagenesis and the metabolism of alpha-ketoglutarate, particularly the role this organic acid plays in stimulating ammonia formation from glutamate, is another investigative focus. Experiments will be performed in rat kidney cortex slices, isolated renal tubules, kidney mitochondria, and the isolated perfused kidney. A unique feature of the project is the utilization of 15N as a tracer for renal nitrogen metabolism and the utilization of gas chromatography-mass spectrometry to quantitate 15N levels in this tissue. The data so generated will be of scientific value in improving our understanding of renal nitrogen metabolism.