CONTROL OF PITUITARY GLAND AND PITUITARY TUMOR HORMONES

我们把研究的重点放在组织脂在分泌中的作用。 调节催乳素和生长激素释放的过程。这个 磷脂酰肌醇的周转与催乳素密切相关 释放，因为TRH对两者都有刺激作用，而多巴胺则 抑制性的。磷脂酰肌醇成交量的增加也是 与细胞内钙储存的释放有关， 分泌过程中的必备离子。这些研究将被延长 测定磷脂代谢产物在钙动员中的作用 和荷尔蒙释放。具体地说，二酰基甘油的功能， 花生四烯酸，这些过程中的白三烯将在 细节。我们有初步证据表明对花生四烯酸的刺激 产生和释放是在分泌过程中的一个重要事件 正常的脑垂体细胞，这些研究将扩展到脑垂体 肿瘤细胞。我们有初步证据表明这些肿瘤细胞有一种 他们在处理钙方面的缺陷，这导致了他们的耐火性 到多巴胺能抑制。这种可能性正在通过以下方式解决 通过使用毛霉毒素促进钙吸收的增加， 钙通道激活剂。我们相信，我们可以恢复 多巴胺抑制催乳素的释放。我们推测， 肿瘤垂体细胞对钙的处理异常减少可能 与它们生产花生四烯酸的能力降低有关。这个建筑群 目前正在进行一系列调查。(C)

We have focused our research on the role of tissue lipids in the secretory processes regulating the release of prolactin and growth hormone. The turnover of phosphatidylinositol has been closely linked with prolactin release because TRH is stimulatory to both, whereas dopamine is inhibitory. An increased turnover of phosphatidylinositol is also correlated with the liberation of intracellular stores of calcium, an obligatory ion for the secretory process. These studies will be extended to determine the role of phospholipid metabolites in calcium mobilization and hormone release. Specifically, the function of diacylglycerol, arachidonate, and the leukotrienes in these processes will be examined in detail. We have preliminary evidence that stimulation of arachidonate production and release is an important event in the secretory process in normal pituitary cells, and these studies will be extended to pituitary tumor cells. We do have preliminary evidence that these tumor cells have a defect in their handling of calcium, and this leads to their refractoriness to dopaminergic inhibition. This possibility is being addressed by promoting an increase in calcium uptake through the use of maitotoxin, a calcium channel activator. We believe that we can restore the ability of dopamine to inhibit prolactin release by this agent. We speculate that the abnormally reduced handling of calcium by the tumor pituitary cells may relate to their reduced ability to produce arachidonate. This complex series of investigations is currently being implemented. (C)