TERATOCARCINOMA AND EMBRYONAL TUMORS: SURFACE ANTIGENS

T/t复合体在细胞表面集合的规范中起作用 抗原似乎在控制细胞相互作用和 在早期发育过程中的识别。两项重要的新研究增加 T/T复合体作为这种控制的模型的能力：(1) 与致死突变之一(TW18)相关的抗原(S)已被 在时间和地点上定位于遗传性功能障碍的部位 以及(2)几种不同的t致死基因 作图；它们是非等位基因，代表了一个明显的基因家族传播 17号染色体超过20厘米，H-2异常位于 他们。我们打算研究t突变染色质中的遗传相关基因。 通过利用我们新发现的能力来绘制它们：(1)通过经典 育种试验；(2)利用重组DNA技术。我们的长期合作 目的是了解糖尿病的分子机制和遗传控制。 正常早期发育中的细胞承诺和非承诺 胚胎肿瘤。 T/T复合体的致死基因揭示了一系列 野生型基因在控制早疫病发生中的重要作用 胚胎发育。我们使用了古典孟德尔式的组合 遗传学和分子遗传学提供T区的详细图谱 发现了三个重要的发现：(1) 整个主要组织相容性复合体[MHC]在t-单倍型中是倒置的； (2)t-leatals分布在三个簇中，其中最大的簇 围绕并与MHC混合；以及(3)不同的t基因， 相隔15厘米，可作为顺式/反式的功能单元 暗示遗传可塑性可能在哺乳动物中发挥作用的测试 发展。(CS)

The T/t complex has a role in the specification of sets of cell surface antigens that appear to be important in controlling cell interactions and recognition during early development. Two important new studies increase the power of the T/t complex as a model of this control: (1) the antigen(s) associated with one of the lethal mutations (tw18) has been localized in time and place to the site of genetically caused dysfunction in the mutant embryo; and (2) several different t-lethal genes have been mapped; they are nonallelic and represent an apparent gene family spread over 20 cM of chromosome 17 with H-2 situated anomalously in the middle of them. We intend to study genetically relevant genes in t-mutant chromatin by taking advantage of our new-found ability to map them: (1) by classical breeding experiments; and (2) by recombinant DNA technology. Our long-term objective is to understand the molecular mechanisms and genetic control of cellular commitment in normal early development and noncommitment in embryonal tumors. The lethal genes of the T/t-complex reveal the existence of a series of wild-type genes that have an important role in the control of early embryonic development. We have used a combination of classical Mendelian genetics and molecular genetics to provide a detailed map of the t-region of mouse chromosome 17. Three important findings have emerged: (1) the entire major histocompatibility complex [MHC] is inverted in t-haplotypes; (2) the t-lethals are arranged in three clusters, the largest of which surrounds and is intermingled with the MHC; and (3) different t genes, separated by as much as 15 cM, can act as a functional unit in cis/trans tests implying that genetic plasticity may play a role in mammalian development. (CS)