ROLES OF LAMININ AND ENTACTIN IN CELL ADHESION

层粘连蛋白和粘连蛋白与基底的主要成分 膜。已有文献表明层粘连蛋白介导的细胞 黏附和铺展、轴突生长和神经元存活。 也有证据表明它与轴突有关。 引导、神经肌肉接头的形成、肾脏 分化胚胎的发育、胚胎发生与维持 功能。层粘连蛋白与其受体的相互作用 牵涉到转移过程和认识到 自然杀伤淋巴细胞及其靶细胞。这个 Entactin在基底膜功能中的作用 不太清楚。发现它与层粘连蛋白存在紧密的非共价联系 最近的证据表明，它可能还参与了细胞 依恋。 本提案的目标是：(A)界定#年的结构 细胞附着所需的层粘连蛋白。(B)隔离和 介质层粘连蛋白对细胞表面分子的表征 结合，(C)阐明entactin在基底膜中的作用 起作用，尤其是在细胞附着和(D) 探讨层粘连蛋白结合蛋白的表达调控 大分子。层粘连蛋白和粘连蛋白的初级序列 将通过重组DNA技术来确定。聚变 含有每个分子片段的蛋白质将被产生 用作细胞结合的探针和抗原。多克隆 用融合蛋白产生的单抗和 将使用由层粘连蛋白和粘连蛋白衍生的多肽 以阐明它们的结合域。的长期目标 该项目是为了定义细胞外细胞外 基质影响细胞在转移过程中的行为， 胚胎发生和细胞分化。

Laminin and entactin and major components of basement membranes. It is well documented that laminin mediated cell adhesion and spreading, neurite outgrowth and neuronal survival. There is also evidence to suggest that it is involved in axonal guidance, formation of neuromuscular junctions, kidney development, embryogenesis and maintenance of differentiated functions. The interactions of laminin and its receptor has been implicated in the metastatic process and in the recognition of natural killer lymphocytes and their target cells. The involvement of entactin in the function of basement membranes is less clear. It found in tight non-covalent association with laminin and recent evidence suggests that it may also be involved in cell attachment. The objectives of this proposal are to (a) define the structures in laminin that are required for cell attachment. (b) isolate and characterize the cell surface molecules the mediate laminin binding, (c) clarify the role of entactin in basement membrane function especially with respect to cell attachment and (d) explore the regulation of expression of the laminin binding macromolecules. The primary sequences of laminin and entactin will be determined by recombinant DNA techniques. Fusion proteins containing segments of each molecule will be generated to be used as probes for cell binding and as antigens. Polyclonal and monoclonal antibodies produced with the fusion proteins and polypeptides derived from laminin and entactin will be employed to eluctidate their bindings domains. The long range objective of the project is to define the mechanisms whereby the extracellular matrix influences the behavior of cells during metastasis, embryogenesis and cellular differentiation.