ANTITUMOR AGENTS FROM MARINE ORGANISMS

本研究的目的是鉴定、分离和确定 一种具有细胞毒性和体内抑瘤活性的化合物结构 各种海洋生物。新化合物的主要来源将是 海绵、海鞘和腔肠动物。此外，海洋细菌 从海绵和一些其他海洋来源分离出来的将被培养和 提取以寻找潜在的抗肿瘤化合物。隔离将是 由国家癌症研究所提供的活动测试指导，使用 体内(PS)和/或体外(KB、P388、L1210)测试系统。在一定程度上 将分离出足够数量的可能化合物，以便在 活体活动测试。将寻求提高抗肿瘤活性 通过对分离得到的部分活性化合物进行结构修饰。 各种成熟的现代分离技术将用于 分离化合物，结构将通过单晶x射线确定 分析和/或常规光谱分析方法。的结构 主要目标附带获得的化学上的新化合物 将在可能的范围内进行调查。 这个项目的长期目标是发现临床上有用的 抗癌剂。

The objectives of this research are to identify, isolate and determine the structure of cytotoxic and in vivo tumor inhibitory compounds from a variety of marine organisms. The primary sources for new compounds will be sponges, ascidians, and coelenterates. In addition, marine bacteria isolated from sponges and some other marine sources will be cultured and extracted to search for potential antitumor compounds. Isolation will be guided by activity testing provided by the National Cancer Institute using in vivo (PS) and/or in vitro (KB, P388, L1210) test systems. To the extent possible compounds will be isolated in sufficient quantities to conduct in vivo activity testing. Antitumor activity enhancement will be sought through structural modification of some of the active compounds isolated. A variety of established, modern separation techniques will be used to isolate compounds, and structures will be determined by single cystal x-ray analysis and/or conventional spectroscopic methods. The structures of chemically novel compounds obtained incidental to the primary objective will be investigated to the extent possible. The long term goal of this project is to discover clinically useful anticancer agents.