13TSB_SynBio: Feasibility study on application of synthetic biology approaches to high yield production of Mycosporine-like Amino Acids for use as ...

到达地球的太阳辐射包括红外线(800 Nm)、可见光(PAR，400-750 nm)和紫外线(UVA，320-400 nm)。暴露在紫外线(UV)辐射下对皮肤的损害是一个主要的社会问题，因此消费者对用于阻止紫外线渗透的化妆品有很大的需求。用于阻止紫外线穿透皮肤的材料包括无机金属氧化物颗粒和有机过滤器。目前，有机紫外线吸收化合物是由不可再生的石化产品制造的，因此，从可再生材料开发有机紫外线吸收化合物具有相当大的商业兴趣。Croda希望开发一种可持续的制造工艺，高效、高水平地生产一组天然存在的紫外线吸收化合物，即类霉菌素类氨基酸(MAAs)，用作护肤品的防晒添加剂。目前，MAA用于防晒霜的商业化生产并不可行，因为产量低，而且缺乏与本土MAA生产生物相关的发酵技术。该项目将通过在一种细菌中表达编码已知MAA生物合成途径酶的基因来克服这一限制，这种细菌被广泛用于生产家用和食品产品，并且在安全方面有着良好的记录。

Solar radiation reaching the Earth consists of infrared (>800 nm), visible (PAR, 400-750 nm) and ultraviolet-A (UVA, 320-400 nm). Damage to skin through exposure to ultra violet (UV) radiation is a major societal concern and consequently there is a significant consumer demand for cosmetics formulated to block UV penetration. Materials used to block UV penetration to the skin include inorganic metal oxide particles and organic filters. Currently, organic UV-absorbing compounds are manufactured from non-renewable petrochemicals and, as a result, there is considerable commercial interest in developing organic UV-absorber compounds from renewable materials. Croda wish to develop a sustainable manufacturing process for efficient, high-level production of a natural occurring group of UV-absorbing compounds, namely Mycosporine-like amino acids (MAAs), for use as a sunscreen additive to skin care products. Currently, the commercial production of MAAs for use in sunscreens is not a viable proposition due to the low yield and lack of fermentation technology associated with native MAA-producing organisms. This project will overcome this limitation by expressing genes encoding known MAA biosynthetic pathway enzymes in a bacterium that is widely used in industry to produce house-hold and food products and that has an excellent track record for safety.