MONOCLONAL ANTIBODIES IN THE IMMUNOHISTOCHEMICAL CLASS

这项研究项目的目的是筛选，并 综合检测单抗(MAb) 免疫组织化学，以确定哪些能够 提高组织学诊断的准确性，或者， 那些识别预后标志物的人 意义。也可以从各种外部来源获得单抗 在我们自己的实验室生产的产品将进行广泛的筛选 针对一组正常的人类组织和选定的肿瘤 不同的组织发生和分化程度。冻住 从我们冷冻的肿瘤库中取出的肿瘤将成为“黄金法则” 用于与石蜡包埋组织的比较研究。冻结- 干燥(未固定)组织、乙醇固定组织和福尔马林固定组织 石蜡包埋后，将用每一单抗进行测试。五花八门 免疫组织化学方法将首先应用于 冷冻和石蜡包埋组织，以最大限度地选择 对每个抗原/抗体的最佳处理。抗体 经过严格的筛查显示组织或肿瘤具有歧视性 产能将与更大的一组油井进行测试 根据肿瘤类型的不同，确定合适的肿瘤类型 筛查过程中表现出的特异性。为了方便在中国进行筛选 寻找具有生物学意义的标记，特别是准备好的 含有肿瘤样本的多肿瘤块 将使用临床定义的患者群体。结果是 免疫组织化学研究将与 组织病理学诊断、肿瘤分级、疾病分期、反应 对治疗、生存期和其他预后参数的影响 对所涉及的肿瘤的意义。

The purpose of this research project is to screen, and comprehensively test monoclonal antibodies (Mabs) by immunohistochemistry, in order to identify those capable of increasing the accuracy of histological diagnosis of, alternatively, those which recognize marker substances of prognostic significance. Mabs obtained from various external sources as well as produced in our own laboratory will be extensively screened against a panel of normal human tissues and selected neoplasms of variable histogenesis and degrees of differentiation. Frozen tumors from our frozen tumor bank will serve as the "golden rule" for comparative studies with paraffin embedded tissues. Freeze- dried (unfixed) tissue, ethanol-fixed and formalin-fixed tissues, all embedded in paraffin will be tested with each Mab. Various immunohistochemical methods will be initially employed on frozen and paraffin embedded tissues to maximize selection of the optimal handling for each antigen/antibody. Antibodies which after rigorous screening show tissue or tumor discriminatory capacity will be tested against a larger group of well characterized neoplasms of appropriate type depending on the specificity shown during screening. To facilitate screening in search of markers of biological significance, especially prepared multi-tumor blocks containing samples of neoplasms from clinically-defined patient populations will be used. The results of the immunohistochemical studies will be correlated with the histopathologic diagnosis, tumor grade, stage of disease, response to therapy, survival and other prognostic parameters of specific significance for the neoplasms involved.