MONOCLONAL ANTIBODIES IN CLASSIFICATION OF TUMORS

本研究项目的目的是评价单抗。 能够提高组织病理学诊断的准确性或 确定有意义的预后群体。获得单抗 将从几个来源中筛选出一组正常人 组织和选定的肿瘤具有不同的组织发生和程度 差异化。一堆冷冻的肿瘤在零下80摄氏度 希望之城解剖病理科将是主要来源 材料。此外，石蜡包埋的肿瘤固定在无水酒精中， 缓冲福尔马林和其他固定剂也将进行测试，如果有保证 初步研究，并与冷冻试验结果进行了比较 横断面。透射电子显微镜和电子显微镜 将对诊断困难的选定病例进行免疫化学检查。 各种免疫组织化学方法将首先应用于冰冻 和石蜡包埋的组织固定在各种固定剂中，以最大限度地 为每个抗原/抗体选择最佳处理方式。抗体 在筛查后显示组织或肿瘤的识别能力 将与更大一组特征良好的肿瘤进行测试 适当的类型取决于先前测试所显示的特异性。这个 免疫组织化学研究的结果将与 组织病理学诊断、肿瘤分级、疾病分期、对 治疗、存活率和其他有特殊意义的预后参数 对于所涉及的肿瘤。(2)

The purpose of this research project is to evaluate monoclonal antibodies capable of permitting increased diagnostic accuracy in histopathology or of identifying significant prognostic groups. Monoclonal antibodies obtained from several sources will be screened against a panel of normal human tissues and selected neoplasms of variable histogenesis and degrees of differentiation. A bank of frozen tumors maintained at -80~C at the Division of Anatomic Pathology, City of Hope will be the primary source of material. In addition, paraffin-embedded tumors fixed in absolute alcohol, buffered formalin, and other fixatives will also be tested, if warranted by preliminary study, and compared with the results obtained from frozen sections. Transmission electron microscopy and electron microscopy immunochemistry will be done on selected cases of diagnostic difficulty. Various immunohistochemical methods will be initially employed on frozen and paraffin-embedded tissues fixed in various fixatives to maximize selection of the optimal handling for each antigen/antibody. Antibodies which after this screening show tissue or tumor discriminatory capacity will be tested against a larger group of well-characterized neoplasms of appropriate type depending on the specificity shown by prior tests. The results of the immunohistochemical studies will be correlated with the histopathologic diagnosis, tumor grade, stage of disease, response to therapy, survival, and other prognostic parameters of specific significance for the neoplasms involved. (2)