NUCLEAR ANTIGEN MARKERS IN HUMAN BLOOD CELLS

抗人血细胞核材料抗血清筛选实验 揭示了几种核抗原在人类细胞中的特殊分布 代表血细胞发育进展阶段的线条。一 MR=55000的突出抗原只存在于一种细胞系HL-60中， 代表了髓系细胞发育的最新阶段。虽然这件事 使用免疫化学结合 蛋白质印迹技术，它只存在于微量的 通过常规蛋白质染色确定的表达细胞 方法：研究方法。使用单特异性抗体显示明显的 最初观察到的特异性不仅仅是由于抗原的改变。 无反应细胞的电泳性迁移特征。这个 通过生化方法确定的核位置得到确认 通过免疫细胞化学。抗原在正常人标本中的定位 骨髓、白血病细胞系和外周血细胞表明细胞核 许多正常的髓系细胞和外周血单核细胞都含有这个 抗原。只有能够在体外诱导成熟的细胞系； 表达了这种抗原。在这些研究中，对细胞类型的调查 分发范围将扩大到包括正常和病理的使用 血液和骨髓样本。其目的将是确定在 在分化过程中，这种抗原的表达开始并 评估其存在在诊断中的有用性。溶解度 属性、染色质中的分布、结合属性和 显微注射抗原的效果将被确定为尝试 一种可能的功能。这些研究将通过生产促进 以及对单抗的鉴定。检测到MR= 某些血细胞中的55,000个抗原表明它可能用作标记物 在血液病理学中，并作为了解核功能的线索。 据信，在原子核中运行的特定机制是 正常的细胞分化过程。中的异常现象 分化和发育在许多人类疾病中起作用。 正常分化中所涉及的成分识别和 阐明它们的作用机制将是 提供预防和干预所需的信息。(MI)

Screening experiments with antisera to human blood cell nuclear materials revealed a specific distribution of a few nuclear antigens in human cell lines representative of progressive stages of blood cell development. One prominent antigen of Mr = 55,000 was present only in one cell line, HL-60, representing the latest stages of myeloid cell development. Although this activity was easily detected using immunochemistry combined with the protein blotting technique, it is present in only small amounts in expressing cells as determined through conventional protein staining methods. Use of monospecific antibodies showed that the apparent specificity initially observed was not simply due to altered antigen electrophoretic migration characteristics in nonreacting cells. The nuclear location determined through the biochemical approach was confirmed by immunocytochemistry. Localization of the antigen in specimens of normal bone marrow, leukemic cell lines, and peripheral blood suggests that nuclei of many normal myeloid cells and peripheral blood monocytes contain this antigen. Only the cell lines capable of being induced to mature in vitro; expressed this antigen. In these studies, the investigation of cell type distribution will be extended to include the use of normal and pathologic blood and bone marrow specimens. The purpose will be to determine where in the differentiation schedule the expression of this antigen begins and to evaluate the usefulness of its presence in diagnosis. The solubility properties, the distribution in chromatin, binding properties, and the effects of microinjected antigen will be determined in an attempt to assign a possible function. These studies will be facilitated by the production and characterization of monoclonal antibodies. The detection of an Mr = 55,000 antigen in certain blood cells suggests its possible use as a marker in hematopathology and as a clue to understanding nuclear function. Specific mechanisms operating in the nucleus are believed to underlie the normal process of cellular differentiation. Abnormalities in differentiation and development have a role in a number of human diseases. The recognition of components involved in normal differentiation and the elucidation of their mechanisms of action will be an important step in providing the information necessary for prevention and intervention. (MI)