TUMOR-ASSOCIATED DNA MOVEMENT

越来越多的证据表明，肿瘤的发生可能需要特定的基因 一些事件，其中一些可能涉及染色体易位。我们有 研究发现，在许多小鼠B细胞肿瘤中，一段DNA经常 移位(肿瘤相关重组DNA或TAR DNA)。在两个B细胞中 品系，TAR DNA已经从潜在的活性重组到下游 免疫球蛋白基因启动子。涉及TAR DNA的重组不是 与c-myc基因易位有关的基因也见于许多B细胞肿瘤。焦油 DNA重组在Lambda的生产中被发现得更频繁 B细胞系比产生kappa的肿瘤中更多。可能是因为焦油 重组是B细胞发育过程中的调节事件 成熟。我们将研究TAR DNA重组以阐明 B细胞肿瘤染色体重组的分子机制 并确定这些重组对肿瘤的发生是否重要。 DNA运动可能在转化过程中发挥重要作用，因为 在正常的发育和进化中也是如此。(D)

Increasing evidence suggests that oncogenesis may require specific genetic events, some of which may involve chromosomal translocations. We have found that, in many murine B-cell tumors, a segment of DNA is frequently translocated (tumor-associated recombining DNA or tar DNA). In two B-cell lines, tar DNA has recombined downstream from potentially active immunoglobulin gene promoters. Recombinations involving tar DNA are not related to c-myc gene translocations also seen in many B-cell tumors. Tar DNA recombinations are found much more frequently in lambda-producing B-cell lines than in kappa-producing tumors. It may be that tar recombination is a developmentally regulated event during B-cell maturation. We will investigate tar DNA recombination to elucidate the molecular mechanisms underlying chromosomal recombinations in B-cell tumors and to determine if these recombinations are important for tumorigenesis. DNA movement may play a significant role in the transformation process as well as in normal development and evolution. (D)