DIAGNOSIS OF LEUKEMIA (CML) ONCOGENE (C-ABL) EXPRESSION

该项目的目的是将最新进展的成果应用于 分子生物学，特别是细胞癌基因的分子克隆， 癌基因相关多肽的合成及原位染色体 杂交技术在人类疾病诊断、评估和监测中的应用 白血病--尤其是慢性粒细胞白血病(CML)。一部小说8 KB RNA与转化的正常细胞同源物杂交 已在患者中检测到Abelson小鼠白血病病毒(c-abl)基因 CML和t(9，22)易位(Ph‘染色体)。它不存在 在无t(9，22)或其他白血病的CML中，极少数例外。我们 将分析慢性粒细胞白血病和相关骨髓增生性疾病的患者 (pH‘阴性CML)，用于这种新的c-ABL RNA。其目标是定义 更准确地说，Ph‘阴性白血病与c-abl表达相关 与疾病阶段和治疗反应有关。这些RNA研究将是 补充c-ABL相关患者的分析和原位 使用v-abl探针进行染色体杂交。这些研究将允许 对慢性粒细胞白血病和相关人类白血病的更精确的(分子)定义。(6)

The object of this project is to apply results of recent advances in molecular biology, specifically molecular cloning of cellular oncogenes, synthesis of oncogene related peptides, and in situ chromosome hybridization to the diagnosis, evaluation, and monitoring of human leukemias--specifically to chronic myelogenous leukemia (CML). A novel 8 Kb RNA hybridizing with the normal, cellular homologue of the transforming gene of Abelson murine leukemia virus (c-abl) has been detected in patients with CML and the t(9,22) translocation (Ph' chromosome). It is not present in CML without the t(9,22) or other leukemias with rare exceptions. We will analyze patients with CML and related myeloproliferative disorders (Ph' negative CML) for this novel c-abl RNA. The objective is to define more precisely the Ph' negative leukemia and to correlate c-abl expression with disease phase and response to therapy. These RNA studies are to be complemented with analysis of c-abl related patients and by in situ chromosome hybridization using a v-abl probe. These studies will allow a more precise (molecular) definition of CML and related human leukemias. (6)