REGULATION OF THE HUMAN THY-1 GENE

THY-1是表达的细胞表面分化标志物 主要存在于许多哺乳动物的大脑中，包括 天哪。在一些物种中，特别是大鼠和小鼠。THY-1也是 在淋巴组织上表达。它的优惠表现在 突触及其在形态发育过程中的出现 大脑表明，它可能在突触的形成中发挥作用。 THY-1的组织特异性表达及其在肿瘤中的变化 不同物种之间的表达模式使Thy-1成为 研究基本机制的理想模型 这是基因调控的基础。我们建议对DNA序列进行识别 以组织特异性的方式调节Thy-1基因。 重组DNA构建物将用于瞬变和稳定 转型系统。此外，转基因小鼠表达 人类Thy-1基因将被用来研究这种机制 Thy-1的不同表达模式在卵巢癌中的作用 不同的物种。

Thy-1 is a cell surface differentiation marker expressed predominantly in the brain of many mammalian species including man. In some species, notably rats and mice. Thy-1 is also expressed on lymphoid tissue. Its preferential expression on synapses and its appearance during morphologic development of the brain suggest that it may play a role in synapse formation. The tissue-specific expression of Thy-1 and its variation in patterns of expression among different species make Thy-1 an ideal model for studying the fundamental mechanisms that underlie gene regulation. We propose to identify DNA sequences that regulate the Thy-1 gene in a tissue-specific manner. Recombinant DNA constructs will be used in transient and stable transformation systems. In addition, transgenic mice expressing the human Thy-1 gene will be used to study the mechanisms responsible for the differing patterns of Thy-1 expression in different species.